TRPM 2 and TRPM 7: Expression Patterns in CNS Cells After Oxygen-Glucose Deprivation

We should have graduate students rewrite these while they still think like the rest of humanity. From the 2011 Canadian Stroke Congress.
Page 81 here:
http://www.strokecongress.org/2011/wp-content/uploads/2011/12/CSC_Abstracts.pdf
TRPM7 and TRPM2 have previously been implicated as regulators of stroke-induced neuronal death. Since, other cation channels implicated in regulating stroke-induced cell death are highly enriched in cortical neurons, we sought to determine the expression of TRPM2 and TRPM7 in various CNS regions over perinatal development
and in specific cell types. Expression patterns were examined in age-specific rat brain tissues and cell-specific primary cultures (enriched neuronal, microglial and astrocyte cultures) using TaqMan real-time PCR to measure dynamic changes in mRNA levels and western blot analyses to quantify protein levels. Furthermore, cell-specific
cultures were subjected to oxygen-glucose deprivation (OGD), and TRP mRNA and protein levels were determined.
Our results indicated that TRPM7 and TRPM2 expression decreased as animals aged. TRPM2 was found to be differentially expressed between cell types, present only in microglia but not cortical neurons or astrocytes, whereas, TRPM7 was expressed at similar levels in all cell types. Also, after a 60 minute period of OGD, TRP levels
had increased. Increased TRP expression after OGD supports previous research implicating TRPM2 and TRPM7 in stroke. Our study also suggests an important role for glial TRPM2, which might inform future development of cell-specific pharmacological inhibitors of TRPM channels.