Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals

I think this is more proof that coffee slows down the progression of dementia. But what the hell do I know? Your doctor is supposed to know all this stuff so ask her/him.
http://www.sciencedirect.com/science/article/pii/S0027510708002595

• Eun Sun Cho^{a, 1},
• Young Jin Jang^{a, 1},
• Mun Kyung Hwang^{a, b},
• Nam Joo Kang^{a, b},
• Ki Won Lee^{b, ,} ,
• Hyong Joo Lee^{a, ,}

• ^a Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea
• ^b Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea

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Abstract

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H₂O₂). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5 μg/ml) or CGA (1 and 5 μM) attenuated H₂O₂-induced PC12 cell death. H₂O₂-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H₂O₂-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X_L and caspase-3. The accumulation of intracellular ROS in H₂O₂-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H₂O₂ in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H₂O₂-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.