Cortical neurogenesis in adult rats after ischemic brain injury: most new neurons fail to mature

Well shit, I was planning on using this to help my recovery. Whom do we have to contact to get this added to the stroke strategy and get human research done on this? Or will this fall thru the cracks like every other interesting stroke research because we have craptastic stroke associations?
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=2;spage=277;epage=285;aulast=Li

Qing-quan Li¹, Guan-qun Qiao¹, Jun Ma¹, Hong-wei Fan², Ying-bin Li^1
1 Department of Neurosurgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China
² Department of Neurosurgery, the First Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China

Date of Acceptance	13-Oct-2014
Date of Web Publication	18-Mar-2015

Correspondence Address:
Ying-bin Li
Department of Neurosurgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province
China

DOI: 10.4103/1673-5374.152383

Abstract

The present study examines the hypothesis that endogenous neural progenitor cells isolated from the neocortex of ischemic brain can differentiate into neurons or glial cells and contribute to neural regeneration. We performed middle cerebral artery occlusion to establish a model of cerebral ischemia/reperfusion injury in adult rats. Immunohistochemical staining of the cortex 1, 3, 7, 14 or 28 days after injury revealed that neural progenitor cells double-positive for nestin and sox-2 appeared in the injured cortex 1 and 3 days post-injury, and were also positive for glial fibrillary acidic protein. New neurons were labeled using bromodeoxyuridine and different stages of maturity were identified using doublecortin, microtubule-associated protein 2 and neuronal nuclei antigen immunohistochemistry. Immature new neurons coexpressing doublecortin and bromodeoxyuridine were observed in the cortex at 3 and 7 days post-injury, and semi-mature and mature new neurons double-positive for microtubule-associated protein 2 and bromodeoxyuridine were found at 14 days post-injury. A few mature new neurons coexpressing neuronal nuclei antigen and bromodeoxyuridine were observed in the injured cortex 28 days post-injury. Glial fibrillary acidic protein/bromodeoxyuridine double-positive astrocytes were also found in the injured cortex. Our findings suggest that neural progenitor cells are present in the damaged cortex of adult rats with cerebral ischemic brain injury, and that they differentiate into astrocytes and immature neurons, but most neurons fail to reach the mature stage.