Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration

We need axon growth so DEMAND your doctor run a clinical trial to see if this would work in humans and what the dosing would be.
http://link.springer.com/article/10.1007/s12035-015-9394-9

• Wang Dingyu,
• Meng Fanjie,
• Ding Zhengzheng,
• Huang Baosheng,
• Yang Chao,
• Pan Yi,
• Wu Huiwen,
• Guo Jun,
• Hu Gang

$39.95 / €34.95 / £29.95 *

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Intracellular tension is the most important characteristic of neuron polarization as well as the growth and regeneration of axons, which can be generated by motor proteins and conducted along the cytoskeleton. To better understand this process, we created Förster resonance energy transfer (FRET)-based tension probes that can be incorporated into microfilaments to provide a real-time measurement of forces in neuron cytoskeletons. We found that our probe could be used to assess the structural tension of neuron polarity. Nerve growth factor (NGF) upregulated structural forces, whereas the glial-scar inhibitors chondroitin sulfate proteoglycan (CSPG) and aggrecan weakened such forces. Notably, the tension across axons was distributed uniformly and remarkably stronger than that in the cell body in NGF-stimulated neurons. The mechanosensors talin/vinculin could antagonize the effect of glial-scar inhibitors via structural forces. However, E-cadherin was closely associated with glial-scar inhibitor-induced downregulation of structural forces. Talin/vinculin was involved in the negative regulation of E-cadherin transcription through the nuclear factor-kappa B pathway. Collectively, this study clarified the mechanism underlying intracellular tension in the growth and regeneration of axons which, conversely, can be regulated by talin and E-cadherin.