The satiating hormone amylin enhances neurogenesis in the area postrema of adult rats

Now if someone would just write up a stroke protocol on all the ways to drive neurogenesis.  A great stroke association president would assign this task to one of the translational researchers on staff. but this won't occur in your lifetime or your childrens lifetime or your grandchildrens lifetime. Survivors are screwed forever.
http://www.sciencedirect.com/science/article/pii/S2212877816300783

• Claudia G. Liberini^{1, 2, 3},
• Tito Borner^{1, 2},
• Christina N. Boyle^{1, ,} ,
• Thomas A. Lutz^{1, 2}

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doi:10.1016/j.molmet.2016.06.015

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Highlights

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Acute amylin affected genes involved in pathways and processes that drive neurogenesis.

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Chronic amylin increased the number of newly proliferating cells in the AP of adult rats.

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Chronic amylin determined neuronal fate over astrocytes in the AP of adult rats.

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Abstract

Objective

Adult neurogenesis in the subgranular zone and subventricular zone is generally accepted, but its existence in other brain areas is still controversial. Circumventricular organs, such as the area postrema (AP) have recently been described as potential neurogenic niches in the adult brain. The AP is the major site of action of the satiating hormone amylin. Amylin has been recently shown to promote the formation of neuronal projections originating from the AP in neonatal rodents but the role of amylin in adult neurogenesis remains unknown.

Methods

To test this, we first performed an RNA-sequencing of the AP of adult rats acutely injected with either amylin (20 μg/kg), amylin plus the amylin receptor antagonist AC187 (500 μg/kg) or vehicle. Second, animals were subcutaneously equipped with minipumps releasing either amylin (50 μg/kg/day) or vehicle for 3 weeks to assess cell proliferation and differentiation with the 5′-bromo-2-deoxyuridine (BrdU) technique.

Results

Acute amylin injections affected genes involved in pathways and processes that control adult neurogenesis. Amylin consistently upregulated NeuroD1 transcript and protein in the adult AP, and this effect was blocked by the co-administration of AC187. Further, chronic amylin treatment increased the number of newly proliferated AP-cells and significantly promoted their differentiation into neurons rather than astrocytes.

Conclusion

Our findings revealed a novel role of the satiating hormone amylin in promoting neurogenesis in the AP of adult rats.