A Novel Short Isoform of Cytosolic PSD-95 Interactor (Cypin) Regulates Neuronal Development

We need dendritic branching post stroke so go ask your doctor what researchers they are working with to bring this research to translational intervention. Doing nothing should result in being fired.
https://link.springer.com/article/10.1007/s12035-017-0849-z

• Authors
• Authors and affiliations

• Mihir V. Patel
• Przemyslaw Swiatkowski
• Munjin Kwon
• Ana R. Rodriguez
• Keith Campagno
• Bonnie L. FiresteinEmail author

• Mihir V. Patel
  • 1
  • 2
• Przemyslaw Swiatkowski
  • 1
  • 3
• Munjin Kwon
  • 1
  • 3
• Ana R. Rodriguez
  • 1
  • 4
• Keith Campagno
  • 1
• Bonnie L. Firestein
  • 1
  Email author

1. 1.Department of Cell Biology and NeuroscienceRutgers University (USA)PiscatawayUSA
2. 2.Graduate Program in NeurosciencesRutgers University (USA)New BrunswickUSA
3. 3.Graduate Program in Molecular BiosciencesRutgers University (USA)New BrunswickUSA
4. 4.Graduate Program in Biomedical EngineeringRutgers University (USA)New BrunswickUSA

Article

First Online: 02 January 2018

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Abstract

The guanine deaminase cypin (cytosolic PSD-95 interactor) binds to PSD-95 (postsynaptic density protein 95) and regulates dendrite branching by promoting microtubule polymerization. Here, we identify a novel short isoform of cypin, termed cypinS, which is expressed in mouse and human, but not rat, tissues. Cypin and cypinS mRNA and protein levels peak at P7 and P14 in the mouse brain, suggesting a role for these isoforms during development. Interestingly, although cypinS lacks guanine deaminase activity, overexpression of cypinS increases dendrite branching. This increase occurs further away from soma than do increases resulting from overexpression of cypin. In contrast, overexpression of cypin, but not cypinS, decreases dendritic spine density and maturity. This suggests that changes to spines, but not to dendrites, may be dependent on guanine deaminase activity. Furthermore, overexpression of either cypin or cypinS increases miniature excitatory postsynaptic current (mEPSC) frequency, pointing to a presynaptic role for both isoforms. Interestingly, overexpression of cypinS results in a significantly greater increase in frequency than does overexpression of cypin. Thus, cypin and cypinS play distinct roles in neuronal development.

Keywords

Cypin Neuron Dendrite Dendritic spine Morphology Electrophysiology Cell culture 

Electronic supplementary material

The online version of this article (https://doi.org/10.1007/s12035-017-0849-z) contains supplementary material, which is available to authorized users.