Repairing the Brain by SCF+G-CSF Treatment at 6 Months Postexperimental Stroke

Now we just need to update and execute that stroke strategy and run human clinical trials on this. Followup which will never occur under current stroke leadership. It will fall thru the cracks like thousands of other studies. Incompetence reigns supreme in the stroke medical world.
Repairing the Brain by SCF+G-CSF Treatment at 6 Months Postexperimental Stroke

Mechanistic Determination of the Causal Link Between Neurovascular Regeneration and Motor Functional Recovery

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Lili Cui, Dandan Wang, Sandra McGillis, ...

First Published August 9, 2016 Research Article

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Abstract

Stroke, a leading cause of adult disability in the world, is a severe medical condition with limited treatment. Physical therapy, the only treatment available for stroke rehabilitation, appears to be effective within 6 months post-stroke. Here, we have mechanistically determined the efficacy of combined two hematopoietic growth factors, stem cell factor (SCF) and granulocyte-colony stimulating factor (G-CSF; SCF + G-CSF), in brain repair 6 months after cortical infarct induction in the transgenic mice carrying yellow fluorescent protein in Layer V pyramidal neurons (Thy1-YFP-H). Using a combination of live brain imaging, whole brain imaging, molecular manipulation, synaptic and vascular assessments, and motor function examination, we found that SCF + G-CSF promoted mushroom spine formation, enlarged postsynaptic membrane size, and increased postsynaptic density-95 accumulation and blood vessel density in the peri-infarct cavity cortex; and that SCF + G-CSF treatment improved motor functional recovery. The SCF + G-CSF-enhanced motor functional recovery was dependent on the synaptic and vascular regeneration in the peri-infarct cavity cortex. These data suggest that a stroke-damaged brain is repairable by SCF + G-CSF even 6 months after the lesion occurs. This study provides novel insights into the development of new restorative strategies for stroke recovery.