Exposing Cells to the Conditions of Ischemia-Reperfusion While Oxygenation is Monitored by the CLARIOstar with ACU

Now if we could just get our researchers to determine the EXACT amount of time that reperfusion needs to occur to leave no lasting damage. With that knowledge we then have a timeframe to shoot for in tPA delivery. Right now all we know is faster is better. That is fucking useless for solving stroke. We need objective endpoints.
https://www.news-medical.net/whitepaper/20180730/Exposing-Cells-to-the-Conditions-of-Ischemia-Reperfusion-While-Oxygenation-Is-Monitored-by-the-CLARIOstar-with-ACU.aspx

• Download Brochure
• Download PDF Copy
• View Supplier Profile
• Request Further Information

Sponsored Content by BMG LABTECH GmbHJul 30 2018

Life-threatening diseases such as myocardial infarction, stroke, or renal failure can occur due to interrupted oxygen flow. Cells and tissues are temporarily deprived of O₂ (ischemia) and develop survival-strategies.
Additionally, upon re-oxygenation (reperfusion), cells are exposed to an additional threat by sudden oxygen increase. Despite being critical for survival, sudden reperfusion of tissue creates an inflammatory response and oxidative stress..
A trial set-up which mimics rapid fluctuations in O₂ level is needed for assessment of biological effects of ischemia-reperfusion.
A microplate reader with software-regulated CO₂ and O₂ flow is employed in the ischemia-reperfusion model shown here. Fluctuations in oxygenation of Cor.4U^® cardiomyocytes and HepG2 cells were recorded using an intracellular oxygen-sensitive fluorescent probe .

Read the full article including the methods, results and discussion.

The CLARIOstar^® with ACU exposes cells to ischemiareperfusion conditions and monitors their oxygenation