Wednesday, May 6, 2020

Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients

Well, they found something promising but didn't complete enough research to prove it out for sure or create protocols on it.

Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients

Natalia Cichon 1,
Ewelina Synowiec 2,
Elzbieta Miller 3,
Tomasz Sliwinski 2,
Michal Ceremuga 4,
Joanna Saluk-Bijak 5, 
Michal Bijak 1,*
1 Biohazard Prevention Centre, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland; natalia.cichon@biol.uni.lodz.pl
2 Department of Molecular Genetics, Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Pomorska 141/143, Poland; ewelina.synowiec@biol.uni.lodz.pl (E.S.), tomasz.sliwinski@biol.uni.lodz.pl (T.S.)
3 Department of Neurological Rehabilitation, Medical University of Lodz, Milionowa 14,  93-113 Lodz, Poland; elzbieta.dorota.miller@umed.lodz.pl
4 Military Institute of Armament Technology, Prymasa Stefana Wyszyńskiego 7, 05-220 Zielonka, Poland ceremugam@witu.mil.pl
5 Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Pomorska 141/143, Poland; joanna.saluk@biol.uni.lodz.pl * Correspondence: michal.bijak@biol.uni.lodz.pl; Tel./Fax: +48-42-635-43-36 Received: 02 April 2020; Accepted: 24 April 2020; Published: 30 April 2020

Abstract: 

Apoptosis in acute stroke is associated with a negative prognosis and is correlated with the severity of the neurological deficit. However, there is no evidence that indicates that, in the subacute phase of the stroke, the apoptosis process might activate neuroplasticity. Therefore, in this study, we investigated the effect of an extremely low frequency electromagnetic field (ELF-EMF) on the molecular mechanism of apoptosis, as used in the rehabilitation of post-stroke patients. Patients with moderate stroke severity (n = 48), 3–4 weeks after incident, were enrolled in the analysis and divided into ELF-EMF and non-ELF-EMF group. The rehabilitation program in both groups involves the following: kinesiotherapy—30 min; psychological therapy—15 min; and neurophysiological routines—60 min. Additionally, the ELF-EMF group was exposed to an ELFEMF (40 Hz, 5 mT). In order to assess the apoptosis gene expression level, we measured the mRNA expression of BAX, BCL-2, CASP8, TNFα, and TP53. We found that ELF-EMF significantly increased the expression of BAX, CASP8, TNFα, and TP53, whereas the BCL-2 mRNA expression after ELFEMF exposition remained at a comparable level in both groups. Thus, we suggest that increasing the expression of pro-apoptotic genes in post-stroke patients promotes the activation of signaling pathways involved in brain plasticity processes. However, further research is needed to clarify this process.

No comments:

Post a Comment