Curcumin promotes neurogenesis of hippocampal dentate gyrus via Wnt/β-catenin signal pathway following cerebral ischemia in mice

In mice so your hospital is responsible for getting research done in humans.  After that they need to have a protocol that will migrate those new neurons to where they are needed.

Curcumin promotes neurogenesis of hippocampal dentate gyrus via Wnt/β-catenin signal pathway following cerebral ischemia in mice

Author links open overlay panelXuemeiYang^bc1DanSong^ad1LiliChen^adHuanXiao^cXiaojiaoMa^cQingsongJiang^c

OumeiCheng^a

https://doi.org/10.1016/j.brainres.2020.147197Get rights and content

Highlights

•

Curcumin ameliorates acute cerebral ischemia-induced cognitive dysfunction in mice.

•

Curcumin promotes hippocampal NSCs proliferation and differentiation into neurons.

•

Curcumin promotes hippocampal neurogenesis through Wnt/β-catenin signaling pathway.

Abstract

Objectives

To investigate whether curcumin promotes hippocampal neurogenesis in the cerebral ischemia (CI) mice via Wnt/β-catenin signaling pathway.

Methods

Male C57BL/6 mice were randomly divided into groups: sham operation group (Sham), cerebral ischemic group (CI), curcumin treatment group (50, 100 mg/kg/d, i.p.) and curcumin (100 mg/kg/d) + DKK1 (a blocker of Wnt receptor, 200 ng/d, icv) group. CI was induced by bilateral common carotid arteries occlusion (BCCAO) for 20 minutes. The Morris water maze test was conducted to detect spatial learning and memory. Immunofluorescence staining was used to examine the proliferation and differentiation of immature neurons in the hippocampal dentate gyrus. The proteins involved in neurogenesis and Wnt signaling pathway were examined using Western blot assay.

Results

Curcumin significantly alleviated cognitive deficits induced by CI. Curcumin dose-dependently increased the proliferation of neural stem cells and promoted the differentiation and maturation of newly generated neural cells into neurons. Curcumin also increased the expression of proteins involved in neurogenesis (including Ngn2, Pax6 and NeuroD 1) and the Wnt/β-catenin signaling pathway. Moreover, the forenamed effects of curcumin were abolished by pretreatment with DKK1, a blocker of Wnt receptor.

Conclusion

Curcumin promotes hippocampal neurogenesis by activating Wnt/β-catenin signaling pathway to ameliorate cognitive deficits after acute CI.