Effectiveness of Injection of Ginkgo biloba Leaf Extract Combined with Early Rehabilitation on Functional Recovery in Patients Following Ischemic Stroke

 Didn't your doctor and hospital put together a protocol on these for your recovery? I guess not; their incompetence is immense.

• NF-κB signaling pathway (5 posts to November 2012)

• Notch1 signaling (3 posts to August 2018)

Effectiveness of Injection of Ginkgo biloba Leaf Extract Combined with Early Rehabilitation on Functional Recovery in Patients Following Ischemic Stroke 

Buy Article:

$107.14 + tax (Refund Policy)

1

Authors: Hong, Qing; Ye, Junqiang; Wang, Xijia; Zhang, Chao

Source: Journal of Biomaterials and Tissue Engineering, Volume 11, Number 2, February 2021, pp. 271-275(5)

Publisher: American Scientific Publishers

DOI: https://doi.org/10.1166/jbt.2021.2543

• < previous article
• |
• view table of contents
• |
• next article >

• Abstract
• References
• Citations
• Supplementary Data
• Suggestions

Background: 

 

The purpose of this study was to investigate whether Gastrodin can activate the Notch 1 signaling pathway in the ischemic brain area to produce neuroprotective effects against cerebral ischemia-reperfusion injury, and to elucidate the role of Notch 1 and NF-κB signaling pathways in the Gastrodin-induced cerebral ischemic tolerance. 

 

Material and methods: 

 

The focal cerebral ischemia reperfusion model of middle cerebral artery embolism was established. TTC staining was applied to detect cerebral infarction. Tunel/NeuN immunofluorescence double labeling was employed to detect apoptosis. WB was used to detect the expressions of proteins related to the Notch 1 and NF-κB pathways.  

 

Results: 

 

Gastrodin can reduce neuron apoptosis in hippocampus after MCAO/R injury. After DAPT blocked Notch 1 signaling, the neuroprotective effects of Gastrodin improving neural function score, reducing cerebral infarction volume, and inhibiting neuronal apoptosis, were all reversed. Compared with the MCAO/R group, DAPT blocking Notch 1 signaling can also improve the neurological score of rats after MCAO/R injury, reduce cerebral infarct volume, and reduce neuronal apoptosis. Gastrodin can activate Notch 1 and NF-κB signaling pathways in cerebral ischemic areas and increase the expression of related proteins. After DAPT inhibited the Notch 1 signaling in the ipsilateral brain region, the phosphorylation level was significantly decreased, indicating that the activity of the NF-κB pathway was regulated by the Notch 1 signaling.  

 

Conclusion: 

 

Gastrodin-mediated protection against cerebral ischemia-reperfusion injury is related to the activation of Notch 1 signaling and the up-regulation of NF-κB signaling pathway activity in neurons of ischemic brain area.

Keywords: Cerebral Ischemia-Reperfusion Injury; Gastrodin; NF-κB; Notch 1