Alzheimer's
disease (AD), classified as neurodegenerative disorder that progresses
over a period of time, is characterized by intracellular neurofibrillary
tangles and extracellular amyloid plaques. This present research work
was designed to develop a polyherbal gel for the treatment of AD. This
research study is aimed to confirm the impact and validation of
polyherbal gel on tauopathy and neurodegeneration that had been induced
by intraperitoneal trimethyltin (TMT) injection to rats.
Polyherbal
loaded gel was prepared by cold method, and characterized for gel
strength, viscosity, permeation and pH. Subsequently, 5 marker based
standardized plant materials of Kalyanka ghrita were incorporated in gellan gum and xanthan gum. Finally, an in-vivo investigation employing rats with TMT-induced neurological disease were used to assess the efficacy of the optimized gel.
On
day 7, the Wistar rats received intraperitoneal injections of TMT. From
day 14 to day 35, the corresponding groups received intranasal
administration of polyherbal gel. In addition to the molecular
parameters such as brain acetyl cholinesterase activity, BDNF (Rat brain
derived neurotropic factor), protein phosphatase 2 A, antioxidant
parameters, and oxidative stress markers, the behavioral parameters were
also determined. Studies were conducted on the brain's monoamine levels
and histology.
Results
Higher permeation over the nasal mucosa was demonstrated by the optimized In-situ
polyherbal gel. Significant improvement in cognition was observed from
the reduced escape latency, longer paths, and increased social or novel
object recognition tests post polyherbal gel treatment. A documented
HPLC technique helped in optimization and standardization of the
polyherbal gel. The polyherbal treatment groups exhibited a considerable
rise in the levels of monoamines, including norepinephrine, dopamine,
and 5-hydroxy tryptamine.
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