Use the labels in the right column to find what you want. Or you can go thru them one by one, there are only 31,940 posts. Searching is done in the search box in upper left corner. I blog on anything to do with stroke. DO NOT DO ANYTHING SUGGESTED HERE AS I AM NOT MEDICALLY TRAINED, YOUR DOCTOR IS, LISTEN TO THEM. BUT I BET THEY DON'T KNOW HOW TO GET YOU 100% RECOVERED. I DON'T EITHER BUT HAVE PLENTY OF QUESTIONS FOR YOUR DOCTOR TO ANSWER.
Wednesday, October 22, 2025
Inhibition of microRNA-126-3p attenuates ischemic stroke by targeting endogenous neuroprotective receptor LRP6 in the Wnt/β-catenin signaling pathway
Didn't your competent? doctor do something for your recovery with microRNA years ago? NO? NO followup human testing? NOTHING? FIRE YOUR DOCTOR!
miR-126 regulates LRP6 in the Wnt/β-catenin pathway post-stroke.
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Inhibition of miR-126 reduces ischemic brain injury in mice.
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Increased miR-126 levels enhance neuronal apoptosis after MCAO.
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LRP6 activation restores Wnt/β-catenin signaling and improves outcomes.
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Targeting miR-126 presents a potential therapeutic strategy for stroke.
Abstract
MicroRNA-126 (miR-126) has emerged as a potential key regulator in ischemic stroke. Yet, its exact mechanism of action is still unexplored. This study aims to investigate whether miR-126 targets and regulates the low-density lipoprotein receptor-related protein (LRP6), an important co-receptor in the Wnt/β-catenin signaling pathway, to reduce ischemic brain injury in mice. C57BL/6 J mice were randomly divided into Sham group, Middle Cerebral Artery Occlusion (MCAO) group, MCAO+miR-126 antagomir group, MCAO+ antagomir NC group, MCAO+miR-126 agomir group and MCAO + agomir NC group. The Garcia nervous system score assessed the neurobehavior of the mice. Infarct volume was determined by 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and the extent of cerebral ischemia-reperfusion injury was assessed by Nissl staining. The miR-126 level was detected by real-time PCR, and the apoptosis index was detected by Western blot. A luciferase reporter assay was applied to demonstrate that LRP6 is the target protein of miR-126. The increased expression of miR-126 in MCAO mice inhibits the Wnt/LRP6/β-catenin pathway and enhances neuronal apoptosis. Inhibition of miR-126 alleviates the infarct area, motor ability damage, and apoptosis in MCAO mice. Additionally, Western blot results and luciferase reporter assay showed that inhibition of miR-126 activates the Wnt/β-catenin signaling pathway via LRP6. Our data suggest that inhibition of microRNA-126 attenuates ischemic stroke by targeting endogenous neuroprotective receptor LRP6 in the Wnt/β-catenin signaling pathway.
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