http://www.sciencedirect.com/science/article/pii/S0024320516305008
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Abstract
Aims
This study's purpose was to evaluate the effect of ultrasound in air at a frequency of 0.04 MHz and an intensity of 50 mW/cm2 on neural differentiation of hBM-MSCs in vitro and on neurogenesis in an ischemic stroke model.
Materials and methods
hBM-MSCs
were exposed to 0.04 MHz ultrasound and then compared with no exposed
one in cell morphology, lactate dehydrogenase (LDH) activity, RT-PCR,
and Western blot. In addition, we made stroke model mice by means of the
photothrombosis (PT) method and these models were exposed to 0.04 MHz
ultrasound after hBM-MSCs injection. We compared with sham group in
histological and immunohistochemical analysis and western blot.
Key findings
Ultrasound
induced neural differentiation without cell death. In stroke models,
inflammatory cells were observed around the infarct area in the Cell,
Cell/Ultrasound group and the brain infarct volume in the
Cell/Ultrasound group was smaller than in the sham group. Further, the
expression of neural proteins in the Cell/Ultrasound group was increased
relative to the sham group.
Significance
The
present study showed that ultrasound promotes neural differentiation of
hBM-MSC and neurogenesis in a mouse stroke model. This may be
applicable as a therapeutic device with the aim of inducing neurogenesis
following stroke.
Graphical abstract
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