Good luck trying to find a source for human milk. Or maybe you want your doctor to increase bromodeoxyuridine (BrdU) another way. Your doctor should be fluent in everything in this research.
BrdU (26 posts to May 2011)
Human Milk Oligosaccharide 2′-Fucosyllactose Reduces Neurodegeneration in Stroke Brain
Abstract
2′-Fucosyllactose
(2’-FL) is a major oligosaccharide in human milk and is present at
trace levels in cow milk. 2’-FL reduces inflammation in the
gastrointestinal tract. Its action in the central nervous system has not
been well characterized. The purpose of this study is to determine
2’-FL-mediated neural protection and repair in culture and stroke brain.
In rat primary cortical neuronal cultures, 2’-FL significantly
antagonized N-methyl-D-aspartate (NMDA) or glutamate-mediated changes in
ATP production, MAP2 immunoreactivity, and TUNEL. The influx of Ca++ (Ca++i) was examined in primary cortical neurons expressing GCaMP5, an endogenous calcium probe. NMDA increased Ca++i;
2’-FL significantly attenuated this reaction. In a rat middle cerebral
artery occlusion model of stroke, we found that intracerebroventricular
pretreatment or oral posttreatment with 2’-FL significantly reduced
brain infarction, mitigated microglial activation, improved locomotor
activity, and upregulated brain-derived neurotrophic factor (BDNF)
expression. Post-stroke delivery of 2’-FL increased bromodeoxyuridine
(BrdU) labeling in the perilesioned area. These BrdU cells co-expressed
NeuN, or nestin, or GFAP. Using subventricular Matrigel cultures, we
demonstrated that 2’-FL increased cell migration from subventricular
zone explant. This response was reduced by anti-BDNF blocking antibody.
In conclusion, our data suggest that 2’-FL has neuroprotective action
through inhibition of Ca++i, inflammation, and apoptosis. Posttreatment with 2’-FL facilitates neural repair in stroke brain.
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