Deans' stroke musings: A robust activity marking system for exploring active neuronal ensembles

Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Tuesday, December 13, 2016

A robust activity marking system for exploring active neuronal ensembles

I'm sure absolutely nothing will be done to use this to figure out how neuroplasticity works.
https://www.ncbi.nlm.nih.gov/pubmed/27661450

Sørensen AT¹, Cooper YA¹, Baratta MV², Weng FJ¹, Zhang Y¹, Ramamoorthi K¹, Fropf R^3,^4,⁵, LaVerriere E¹, Xue J¹, Young A¹, Schneider C¹, Gøtzsche CR¹, Hemberg M⁶, Yin JC^3,⁴, Maier SF⁷, Lin Y¹.

Author information

¹McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United States.
²Institute for Behavioral Genetics, University of Colorado Boulder, Boulder, United States.
³Department of Genetics, University of Wisconsin-Madison, Madison, United States.
⁴Department of Neurology, University of Wisconsin-Madison, Madison, United States.
⁵Neuroscience Training Program, University of Wisconsin-Madison, Madison, United States.
⁶Wellcome Trust Sanger Institute, Hinxton, United Kingdom.
⁷Department of Psychology and Neuroscience, University of Colorado Boulder, Boulder, United States.

Abstract

Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system's versatility.