Deans' stroke musings

Changing stroke rehab and research worldwide now.Time is Brain!Just think of all the trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 493 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It's quite disgusting that this information is not available from every stroke association and doctors group.
My back ground story is here:http://oc1dean.blogspot.com/2010/11/my-background-story_8.html

Tuesday, December 13, 2016

A robust activity marking system for exploring active neuronal ensembles


I'm sure absolutely nothing will be done to use this to figure out how neuroplasticity works.
https://www.ncbi.nlm.nih.gov/pubmed/27661450

Author information

  • 1McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United States.
  • 2Institute for Behavioral Genetics, University of Colorado Boulder, Boulder, United States.
  • 3Department of Genetics, University of Wisconsin-Madison, Madison, United States.
  • 4Department of Neurology, University of Wisconsin-Madison, Madison, United States.
  • 5Neuroscience Training Program, University of Wisconsin-Madison, Madison, United States.
  • 6Wellcome Trust Sanger Institute, Hinxton, United Kingdom.
  • 7Department of Psychology and Neuroscience, University of Colorado Boulder, Boulder, United States.

Abstract

Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system's versatility.

KEYWORDS:

D. melanogaster; Npas4; RAM; Tet-Off system; activity mapping; cFos; mouse; neural ensemble; neuroscience; rat
PMID:
27661450
PMCID:
PMC5035142
DOI:
10.7554/eLife.13918
[PubMed - as supplied by publisher]
Free PMC Article

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