The Effects of Resveratrol, Caffeine, β‐Carotene, and Epigallocatechin Gallate (EGCG) on Amyloid‐β25–35 Aggregation in Synthetic Brain Membranes

You'll have to ask you doctor what this means in layperson terms.  Or I guess we could ask our fucking failures of stroke associations. But they know absolutely nothing.

The Effects of Resveratrol, Caffeine, β‐Carotene, and Epigallocatechin Gallate (EGCG) on Amyloid‐β_25–35 Aggregation in Synthetic Brain Membranes

Isabella P. Gastaldo

Sebastian Himbert

Udbhav Ram

Maikel C. Rheinstädter

First published: 27 September 2020

https://doi.org/10.1002/mnfr.202000632

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as https://doi.org/10.1002/mnfr.202000632

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Abstract

Scope: Alzheimer's Disease (AD) is a neurodegenerative condition marked by the formation and aggregation of amyloid‐β (Aβ) peptides. There exists, to this day, no cure or effective prevention for the disease; however, there is evidence that a healthy diet and certain food products can slow down first occurrence and progression of peptide aggregates. To investigate if food ingredients can interact with peptide aggregates in membrane mimics, we prepared synthetic membranes that contained aggregates consisting of cross‐β sheets of Aβ_25–35.

Methods and Results: We studied the impact of resveratrol, found in grapes, caffeine, ingredient in coffee, β‐carotene, found in orange fruits and vegetables, and Epigallocatechin gallate (EGCG), a component of green tea, on the size and volume fraction of Aβ aggregates using optical and fluorescence microscopy, X‐ray diffraction, UV‐Visible spectroscopy, and Molecular Dynamics (MD) simulations. All compounds were found to be membrane active and spontaneously partitioned in the synthetic brain membranes. While resveratrol and caffeine led to membrane thickening and reduced membrane fluidity, β‐carotene and EGCG preserved or increased fluidity.

Conclusion: Resveratrol and caffeine did not reduce the volume fraction of peptide aggregates while β‐carotene significantly reduced plaque size. Interestingly, EGCG dissolved peptide aggregates and significantly decreased the corresponding cross‐β and β‐sheet signals.