Sounds promising. Now we just need our stroke doctors and hospitals to get research done in humans and protocols created.
Cannabidiol confers neuroprotection in rats in amodel of transient global cerebral ischemia: impact of hippocampal synaptic neuroplasticity
Erika Meyer
State University of Maringá Centre of Health Sciences: Universidade Estadual de Maringa Centro de
Ciencias da Saude
Jéssica Mendes Bonato
State University of Maringa Centre for Humanities Letters and Arts: Universidade Estadual de Maringa
Centro de Ciencias Humanas Letras e Artes
Marco Aurélio Mori
State University of Maringá Centre of Health Sciences: Universidade Estadual de Maringa Centro de
Ciencias da Saude
Bianca Andretto Mattos
State University of Maring�: Universidade Estadual de Maringa
Francisco Silveira Guimarães
USP FMRP: Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto
Humberto Milani
State University of Maring�: Universidade Estadual de Maringa
Alline Cristina de Campos
USP FMRP: Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto
Rubia M W Oliveira ( rubiaweffort@gmail.com )
University of Sao Paulo https://orcid.org/0000-0002-6181-1881
Research Article
Keywords: Cannabidiol, transient global cerebral ischemia, memory, hippocampus, neuroplasticity
DOI: https://doi.org/10.21203/rs.3.rs-447933/v1
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Read Full LicensePage 2/29
greatly limited. Spatial/temporal disorientation and cognitive dysfunction are among the most prominent
long-term sequelae of CI. Cannabidiol (CBD) is a non-psychotomimetic constituent of Cannabis sativa
that exerts neuroprotective effects against experimental CI. The present study investigated possible
neuroprotective mechanisms of action of CBD on spatial memory impairments that are caused by
transient global cerebral ischemia (TGCI) in rats. Hippocampal synaptic plasticity is a fundamental
mechanism of learning and memory. Thus, we also evaluated the impact of CBD on neuroplastic
changes in the hippocampus after TGCI. Wistar rats were trained to learn an eight-arm aversive radial
maze (AvRM) task and underwent either sham or TGCI surgery. The animals received vehicle or 10 mg/kg
CBD (i.p.) 30 min before surgery, 3 h after surgery, and then once daily for 14 days. On days 7 and 14, we
performed a retention memory test. Another group of rats that received the same pharmacological
treatment was tested in the object location test (OLT). Brains were removed and processed to assess
neuronal degeneration, synaptic protein levels, and dendritic remodeling in the hippocampus.
State University of Maringá Centre of Health Sciences: Universidade Estadual de Maringa Centro de
Ciencias da Saude
Jéssica Mendes Bonato
State University of Maringa Centre for Humanities Letters and Arts: Universidade Estadual de Maringa
Centro de Ciencias Humanas Letras e Artes
Marco Aurélio Mori
State University of Maringá Centre of Health Sciences: Universidade Estadual de Maringa Centro de
Ciencias da Saude
Bianca Andretto Mattos
State University of Maring�: Universidade Estadual de Maringa
Francisco Silveira Guimarães
USP FMRP: Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto
Humberto Milani
State University of Maring�: Universidade Estadual de Maringa
Alline Cristina de Campos
USP FMRP: Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto
Rubia M W Oliveira ( rubiaweffort@gmail.com )
University of Sao Paulo https://orcid.org/0000-0002-6181-1881
Research Article
Keywords: Cannabidiol, transient global cerebral ischemia, memory, hippocampus, neuroplasticity
DOI: https://doi.org/10.21203/rs.3.rs-447933/v1
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Read Full LicensePage 2/29
Abstract
Evidence for the clinical use of neuroprotective drugs for the treatment of cerebral ischemia (CI) is stillgreatly limited. Spatial/temporal disorientation and cognitive dysfunction are among the most prominent
long-term sequelae of CI. Cannabidiol (CBD) is a non-psychotomimetic constituent of Cannabis sativa
that exerts neuroprotective effects against experimental CI. The present study investigated possible
neuroprotective mechanisms of action of CBD on spatial memory impairments that are caused by
transient global cerebral ischemia (TGCI) in rats. Hippocampal synaptic plasticity is a fundamental
mechanism of learning and memory. Thus, we also evaluated the impact of CBD on neuroplastic
changes in the hippocampus after TGCI. Wistar rats were trained to learn an eight-arm aversive radial
maze (AvRM) task and underwent either sham or TGCI surgery. The animals received vehicle or 10 mg/kg
CBD (i.p.) 30 min before surgery, 3 h after surgery, and then once daily for 14 days. On days 7 and 14, we
performed a retention memory test. Another group of rats that received the same pharmacological
treatment was tested in the object location test (OLT). Brains were removed and processed to assess
neuronal degeneration, synaptic protein levels, and dendritic remodeling in the hippocampus.
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