https://www.ncbi.nlm.nih.gov/pubmed/27484673
Abstract
Photobiomodulation,
also known as low-level laser/light therapy (LLLT), refers to the use
of red-to-near-infrared light to stimulate cellular functions for
physiological or clinical benefits. The mechanism of LLLT is assumed to
rely on photon absorption by cytochrome c oxidase (CCO), the terminal
enzyme in the mitochondrial respiratory chain that catalyzes the
reduction of oxygen for energy metabolism. In this study, we used
broadband near-infrared spectroscopy (NIRS) to measure the LLLT-induced
changes in CCO and hemoglobin concentrations in human forearms in vivo.
Eleven healthy participants were administered with 1064-nm laser and
placebo treatments on their right forearms. The spectroscopic data were
analyzed and fitted with wavelength-dependent, modified Beer-Lambert
Law. We found that LLLT induced significant increases of CCO
concentration (Δ[CCO]) and oxygenated hemoglobin concentration (Δ[HbO])
on the treated site as the laser energy dose accumulated over time. A
strong linear interplay between Δ[CCO] and Δ[HbO] was observed for the
first time during LLLT, indicating a hemodynamic response of oxygen
supply and blood volume closely coupled to the up-regulation of CCO
induced by photobiomodulation. These results demonstrate the tremendous
potential of broadband NIRS as a non-invasive, in vivo means to study
mechanisms of photobiomodulation and perform treatment evaluations of
LLLT.
- PMID:
- 27484673
- PMCID:
- PMC4971496
- DOI:
- 10.1038/srep30540
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