Sleep loss disrupts pericyte-brain endothelial cell interactions impairing blood-brain barrier function

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Sleep loss disrupts pericyte-brain endothelial cell interactions impairing blood-brain barrier function

Author links open overlay panelFernandaMedina-Flores^ab

GabrielaHurtado-Alvarado^aArturoContis-Montes de Oca^cStefaniePaola López-Cervantes^bdMinaKonigsberg^dMaria A.Deli^efBeatrizGómez-González^a

https://doi.org/10.1016/j.bbi.2020.05.077Get rights and content

Highlights

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Sleep loss induces pericyte detachment from capillary walls.

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Sleep loss reduces PDGFR-β and Connexin-43 expression in brain microvessels.

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Pericyte detachment during sleep loss decreases claudin-5 and occludin expression.

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Pericyte detachment during sleep loss increases blood-brain barrier permeability.

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Sleep loss increases MMP-9, p-NFκB, A_2A receptor expression in brain microvessels.

Abstract

Sleep loss in the rat increases blood-brain barrier permeability to circulating molecules by disrupting interendothelial tight junctions. Despite the description of the ultrastructure of cerebral microvessels and the evidence of an apparent pericyte detachment from capillary wall in sleep restricted rats the effect of sleep loss on pericytes is unknown. Here we characterized the interactions between pericytes and brain endothelial cells after sleep loss using male Wistar rats. Animals were sleep-restricted 20 h daily with 4 h sleep recovery for 10 days. At the end of the sleep restriction, brain microvessels (MVs) were isolated from cerebral cortex and hippocampus and processed for Western blot and immunocytochemistry to evaluate markers of pericyte-endothelial cell interaction (connexin 43, PDGFR-β), tight junction proteins, and proinflammatory mediator proteins (MMP9, A_2A adenosine receptor, CD73, NFκB). Sleep restriction reduced PDGFR-β and connexin 43 expression in MVs; in addition, scanning electron microscopy micrographs showed that pericytes were detached from capillary walls, but did not undergo apoptosis (as depicted by a reduced active caspase-3 expression). Sleep restriction also decreased tight junction protein expression in MVs and increased BBB permeability to low- and high-molecular weight tracers in in vivo permeability assays. Those alterations seemed to depend on a low-grade inflammatory status as reflected by the increased expression of phosphorylated NFκB and A_2A adenosine receptor in brain endothelial cells from the sleep-restricted rats. Our data show that pericyte-brain endothelial cell interaction is altered by sleep restriction; this evidence is essential to understand the role of sleep in regulating blood-brain barrier function.