In mice so your stroke hospital needs to engage researchers to do human testing. That will never occur because survivors are not in charge at your hospital.
MiR-34a Interacts with Cytochrome c and Shapes Stroke Outcomes
Abstract
Blood-brain
barrier (BBB) dysfunction occurs in cerebrovascular diseases and
neurodegenerative disorders such as stroke. Opening of the BBB during a
stroke has a negative impact on acute outcomes. We have recently
demonstrated that miR-34a regulates the BBB by targeting cytochrome c
(CYC) in vitro. To investigate the role of miR-34a in a stroke,
we purified primary cerebrovascular endothelial cells (pCECs) from mouse
brains following 1 h transient middle cerebral artery occlusion (tMCAO)
and measured real-time PCR to detect miR-34a levels. We demonstrate
that the miR-34a levels are elevated in pCECs from tMCAO mice at the
time point of BBB opening following 1 h tMCAO and reperfusion.
Interestingly, knockout of miR-34a significantly reduces BBB
permeability, alleviates disruption of tight junctions, and improves
stroke outcomes compared to wild-type (WT) controls. CYC is decreased in
the ischemic hemispheres and pCECs from WT but not in miR-34a−/− mice following stroke reperfusion. We further confirmed CYC is a target of miR-34a by a dural luciferase reporter gene assay in vitro.
Our study provides the first description of miR-34a affecting stroke
outcomes and may lead to discovery of new mechanisms and treatments for
cerebrovascular and neurodegenerative diseases such as stroke.
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