Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Friday, June 10, 2022

Transplantation of layer-by-layer assembled neural stem cells tethered with vascular endothelial growth factor reservoir promotes neurogenesis and angiogenesis after ischemic stroke in mice

WHOM is going to get this into the stroke strategy and ensure this gets tested in humans? Is there any responsible party in stroke actually trying to solve stroke or is every fucking idiot just twiddling their thumbs?

Oops, I'm not playing by the polite rules of Dale Carnegie, 'How to Win Friends and Influence People'.

Telling supposedly smart stroke medical persons they know nothing about stroke is a no-no even if it is true.

Politeness will never solve anything in stroke. Yes, I'm a bomb thrower and proud of it. Someday a stroke 'leader' will try to ream me out for making them look bad by being truthful , I look forward to that day.

Author links open overlay panelHongfeiGe^a QuanHu^b TunanChen^a YangYang^c ChaoZhang^a JunZhong^a YiYin^a XuhengJiang^b XinZhou^b ShuhongWang^b RongHu^a

WenyanLi^a HuaFeng^a

https://doi.org/10.1016/j.apmt.2022.101548 Get rights and content

Highlights

•: LbL assembled NSCs were successfully constructed using gelatin and hyaluronic acid.
•: LbL-NSCs are biocompatible, and hold the ability of promoting NSCs differentiation into neurons.
•: Transplantation of LbL (VEGF)-NSCs promote implanted NSCs survival and differentiation into neurons after dMCAO.
•: Engraftment of LbL (VEGF)-NSCs reinforce neurogenesis, angiogenesis, survival of host neurons, and BBB repair.
•: LbL (VEGF)-NSCs facilitate host neurocytes survival and neuroblasts migration.

Abstract

Transplantation of neural stem cells (NSCs) is a promising therapeutic strategy for ischemic stroke. However, most of engrafted NSCs hardly survive for a long time,(But earlier research suggests it's the exosomes created from these NSCs that create the benefit.) and the majority of transplanted NSCs differentiate into astrocytes around infarct core due to harsh microenvironment after ischemic stroke. Therefore, exploring approaches to foster the regenerative ability of transplanted NSCs in hostile niche is a feasible strategy to rehabilitate lesions. Here, a feasible method for grafted NSCs modified by layer-by-layer (LbL) assembly (LbL-NSCs) using gelatin and hyaluronic acid (HA) was developed. The results indicated the LbL-NSCs were biocompatible, and held the ability of promoting NSCs differentiation into neurons. Subsequently, vascular endothelial growth factor (VEGF) was laden into gelatin to generate LbL-NSCs tethered with VEGF reservoir [LbL (VEGF)-NSCs], and the VEGF releasing from LbL (VEGF)-NSCs was sustained under pH 7.4 in vitro. Afterward, the results demonstrated transplantation of LbL (VEGF)-NSCs facilitated implanted NSCs survival and differentiation into neurons in distal middle cerebral artery occlusion (dMCAO) mice. Additionally, engraftment of LbL (VEGF)-NSCs reinforced neurogenesis, angiogenesis, survival of host neurons, and blood brain barrier (BBB) repair, thereafter enhancing functional recovery through reducing the volume of infarct core in dMCAO mice. This investigation provides an appropriate approach for modifying NSCs by LbL assembly using gelatin loaded with VEGF and HA to expedite the rehabilitative ability of engrafted NSCs following ischemic stroke.

Graphical abstract

Graphical illustration of the fabrication of layer-by-layer (LbL) assembled NSCs tethered with VEGF reservoir [LbL (VEGF)-NSCs], and therapeutic effects of LbL (VEGF)-NSCs on neurovascular network rehabilitation after transplantation in mice with ischemic stroke.