https://cld.pt/dl/download/5cf0dc5f-72b0-4b05-953e-484f624b49f6/MyPapers/l984jnf_95.pdf
Abstract
Icariin is a major component derived
from the traditional Chinese herb
Epimedium brevicornum Maxim. Our
previous studies have shown that icariin protects neurons from the
neurotoxicity and ischemia-conditions. In
this study, we investigated the effect
of icariin on the expression of amyloid precursor protein (APP) and the level
of amyloid-β peptide (Aβ), as well as neurogenesis in the brain of Tg2576 mice,
an animal model of Alzheimer’s disease (AD). Tg2576 mice and wild type
littermates (WT) were randomly assigned to three groups: Tg2576, Tg2576+icariin
and WT groups. At 9 months old, all mice were treated with icariin (60 mg/kg/d)
or distilled
water for 3 months. The results
showed that administration with icariin for 3 months improved the spatial
working memory of Tg2576 mice as examined in Y-maze task. Furthermore, icariin
treatment reduced the level of
insoluble Aβ1-40 (69%) and Aβ1-42
(50%) in the cortex and hippocampus
as determined by ELISA. Western blot analysis indicated the down-regulation of
the APP expression, and double staining showed the elevation of BrdU/NeuN
double-positive cells in the dentate gyrus region of hippocampus compared with
Tg2576 mice. The current study demonstrated that icariin improved memory
function, decreased the levels of Aβ and APP in the brain and increased the
neurogenesis in the hippocampus of Tg2576 mice. Taken together,
these results suggest that icariin
could be a potential drug candidate for AD treatment.
No comments:
Post a Comment