Deans' stroke musings: Researchers at Brigham and Women's Hospital (BWH) have developed a platform technology for monitoring single-cell interactions in real-time

Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Monday, July 25, 2011

Researchers at Brigham and Women's Hospital (BWH) have developed a platform technology for monitoring single-cell interactions in real-time

Tell your researchers that this functionality should be able to map neurons as they try to connect to each other. Watching it in real time would be useful in telling what some of the neuroplasticity and neurogenesis therapies are doing. And once we know that we should be able to come up with specific therapies that work. Heck, we should be able to do this in the next 30 years.
http://nextbigfuture.com/2011/07/researchers-at-brigham-and-womens.html

The ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy.

13 more pages of detail here:
http://www.nature.com/nnano/journal/vaop/ncurrent/extref/nnano.2011.101-s1.pdf
Someone has to push this kind of stuff so contact your researcher and your stroke association and ask them what they are going to do with this information.