dl-3-n-butylphthalide promotes neuroplasticity and motor recovery in stroke rats

Your doctor and hospital better get cracking on followup research in humans for this. With 10s of thousands of hospitals and doctors out there at least one should be able to solve this into a translational result.  But you know that will never occur, it hasn't occurred in the last 50 years it won't miraculously start occurring now.
http://www.sciencedirect.com/science/article/pii/S0166432817303042

• Yefei Sun^a,
• Xi Cheng^b,
• Huibin Wang^b,
• Xiaopeng Mu^b,
• Yifan Liang^b,
• YuJia Luo^b,
• Huiling Qu^c,
• Chuansheng Zhao^{b, ,}

 Show more

https://doi.org/10.1016/j.bbr.2017.04.039

Get rights and content

---

Highlights

•

dl-NBP promoted the behavioral performance after cerebral ischemia in rats.

•

dl-NBP improved the behavioral recovery possibly by increasing neurogenesis, axonal regeneration and synapse formation.

•

The axonal regeneration promoted by dl-NBP may be mediated through the Nogo-A/NgR and RhoA/ROCK pathway.

---

Abstract

Backgrounds and aims

Racemic l-3-n-butylphthalide (dl-NBP), is able to achieve a functional recovery in animal models of cerebral ischemia, vascular dementia, and Alzheimer’s disease. In this study, we investigated the effect of dl-NBP on axonal growth, neurogenesis and behavioral performances in rats with cerebral ischemia.

Methods

Focal cerebral ischemia in rats was produced by intracerebral injection of endothelin-1. Starting from postoperative day 7, the experimental rats were administered 70 mg/kg dl-NBP by oral gavage for two weeks. Biotinylated dextran amine (BDA) was injected into the contralateral sensorimotor cortex on day 14 after ischemia to trace the sprouting of corticospinal tract (CST) fibers into the denervated cervical spinal cord. The expressions of Nogo-A, Nogo-R, Rho-A, and ROCK in the perilesional cortex, the expressions of BDA, PSD-95, and vGlut1 in the denervated spinal cord, 5-bromo-20-deoxyuridine (BrdU)/DCX-positive cells in the subventricular zone (SVZ) of the injured hemisphere were detected by immunofluorescence. The rats’ behavioral abilities were measured on postoperative days 30–32 in the beam-walking, cylinder and sticky label tests.

Results

dl-NBP treatment significantly increased the number and length of crossing CST fibers, enhanced significantly the expression levels of synapse-associated proteins including PSD95 and VGlut-1 in the denervated cervical spinal cord, elevated the number of BrdU+/DCX+ cells in SVZ, and reduced markedly those of Rho-A+, ROCK+, Nogo-A+ and Nogo-R+ cells in perilesional cortex. In addition, dl-NBP improved the behavioral performance of the ischemic rats.

Conclusion

dl-NBP enhanced the behavioral recovery after cerebral ischemia in rats, possibly by increasing axonal growth and neurogenesis.

Keywords

• Axonal growth;
• dl-NBP;
• Neurogenesis;
• Stroke

Choose an option to locate/access this article:

Check if you have access through your login credentials or your institution

Check access

Purchase $41.95

• 

Correspondence to: No.155, North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China.

© 2017 Elsevier B.V. All rights reserved.