Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Saturday, December 16, 2017

Snapshots of Life: Growing Mini-Brains in a Dish

With all this earlier research our researchers should be able to put this all together and create real human brains that can be damaged by stroke and show how to stop and reverse such damage. But that is way too pie in the sky for our stroke medical professionals to understand and implement. 

Multiregional brain on a chip  Jan 2017


 


Draper Laboratory developing “Brain-on-a-Chip”  October 2012


 


"Alzheimer's-in-a-Dish" Docs Win Top Smithsonian Ingenuity Award Nov. 2015 


 


A patient’s budding cortex — in a dish?  June 2015 



Cell cultures in petri dishes open new doors to brain research  April 2017


 


 


 The latest here:

Snapshots of Life: Growing Mini-Brains in a Dish




Brain grown in a lab dish
Credit: Collin Edington and Iris Lee, Department of Biomedical Engineering, MIT
Something pretty incredible happens—both visually and scientifically—when researchers spread neural stem cells onto a gel-like matrix in a lab dish and wait to see what happens. Gradually, the cells differentiate and self-assemble to form cohesive organoids that resemble miniature brains!
In this image of a mini-brain organoid, the center consists of a clump of neuronal bodies (magenta), surrounded by an intricate network of branching extensions (green) through which these cells relay information. Scattered throughout the mini-brain are star-shaped astrocytes (red) that serve as support cells.
Collin Edington and Iris Lee created this striking image in Linda Griffith’s lab at Massachusetts Institute of Technology (MIT), Cambridge. While it looks like a single image, the picture actually took about 12 hours to produce, using a confocal laser scanning microscope to zoom in and capture the microscopic details, pixel by pixel, before digitally stitching the series of images all together. For their painstaking efforts, the team was one of 10 winners in the 2017 Koch Institute Image Awards at MIT, established to recognize and publicly display extraordinary works of art and science.
One of the things that fascinated Edington and Lee about this particular mini-brain was its surprisingly organized structure, which emerged over the course of about two weeks. In fact, one of the most interesting aspects of growing organoids in the lab is that by simply changing the chemical makeup and physical consistency of the hydrogel on which the stem cells are placed, it is possible to influence the ways in which they differentiate and assemble. This enables researchers to tinker with and fine-tune the gel’s characteristics to encourage cells to take on different forms. It also reveals a critical component of the development of complex organs—the cellular environment really matters.
In the experiment captured in this image, the researchers randomly seeded thousands of neural stem cells from human embryonic stem cell lines into a dime-sized well on a standard laboratory plate. Those cells divided and differentiated into hundreds of thousands of cells to form the mini-brain organoid shown above. By studying these mini-brains in the lab, researchers can explore the way a “typical” organoid functions, as well as learn what goes wrong in organoids generated from induced pluripotent stem cells derived from people affected by Alzheimer’s disease or other brain conditions.
Griffith’s lab isn’t stopping with the brain. In fact, this mini-brain is part of a much larger project, funded by NIH and Defense Advanced Research Projects Agency, to produce many different types of “mini-organs”—representing the liver, gut, lung, heart, and more. The goal is to link the individual organoids together to form a “human on a chip.” That integrated approach is important because organs don’t act in isolation in either health or sickness.
Alzheimer’s disease is a good example. While it’s primarily a disease of the brain, it has become increasingly clear that the gut and liver play a role, too. The “human on a chip” platform will make it possible to study such complex interactions in Alzheimer’s disease and many other conditions in the lab, with the ultimate goal to accelerate development of safe and effective new treatments.
Links:
Griffith Lab (MIT, Cambridge, MA)
Meet Chip (National Center for Advancing Translational Sciences/NIH)
NIH Support: National Center for Advancing Translational Sciences



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