http://oto.sagepub.com/content/early/2013/02/02/0194599813475777.abstract
Abstract
Objective Our previous
studies have demonstrated the efficacy of resveratrol, a grape
constituent noted for its antioxidant and anti-inflammatory
properties, in reducing temporary threshold
shifts and decreasing cochlear hair cell damage following noise
exposure. This
study was designed to identify the potential
protective mechanism of resveratrol by measuring its effect on
cyclooxygenase-2
(COX-2) protein expression and reactive oxygen
species (ROS) formation following noise exposure.
Study Design Controlled animal intervention study.
Setting Otology Laboratory, Henry Ford Health System.
Subjects and Methods
Twenty-two healthy male Fischer 344 rats (2-3 months old) were exposed
to acoustic trauma of variable duration with or without
intervention. An additional 20 healthy male rats
were used to study COX-2 expression at different time points during and
following
treatment of 24 hours of noise exposure.
Cochlear harvest was performed at various time intervals for measurement
of COX-2
protein expression via Western blot analysis and
immunostaining. Peripheral blood was also obtained for ROS analysis
using
flow cytometry.
Results Acoustic
trauma exposure resulted in a progressive up-regulation of COX-2 protein
expression, commencing at 8 hours and peaking
at 32 hours. Similarly, ROS production increased
after noise exposure. However, treatment with resveratrol reduced
noise-induced
COX-2 expression as well as ROS formation in the
blood as compared with the controls.
Conclusion COX-2
levels are induced dramatically following noise exposure. This increased
expression may be a potential mechanism of
noise-induced hearing loss (NIHL) and a possible
mechanism of resveratrol’s ability to mitigate NIHL by its ability to
reduce
COX-2 expression.
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