http://www.sciencedirect.com/science/article/pii/S1751616114003130
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Check access- DOI: 10.1016/j.jmbbm.2014.09.029
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Abstract
Despite
technological advances over the past 25 years, a complete recovery from
peripheral nerve injuries remains unsatisfactory today. The autograft
is still considered the “gold standard” in clinical practice; however,
postoperative complications and limited availability of nerve tissue has
motivated the development of alternative approaches. Among them, the
development of biomimetic nerve graft substitutes is one of the most
promising strategies. In this study, multichanneled silk electrospun
conduits bi-functionalized with Nerve Growth Factor (NGF) and Ciliary
Neurotropic Factor (CNTF) were fabricated to enhance peripheral nerve
regeneration. These bioactive guides consisting of longitudinally
oriented channels and aligned nanofibers were designed in order to mimic
the fascicular architecture and fibrous extracellular matrix found in
native nerve. The simple use of the electrospinning technique followed
by a manual manipulation to manufacture these conduits provides
tailoring of channel number and diameter size to create perineurium-like
structures. Functionalization of the silk fibroin nanofiber did not
affect its secondary structure and chemical property. ELISA assays
showed the absence of growth factors passive release from the
functionalized fibers avoiding the topical accumulation of proteins.
Additionally, our biomimetic multichanneled functionalized nerve guides
displayed a mechanical behavior comparable to that of rat sciatic nerve
with an ultimate peak stress of 4.0±0.6 MPa and a corresponding
elongation at failure of 156.8±46.7%. Taken together, our results
demonstrate for the first time our ability to design and characterize a
bi-functionalized nerve conduit consisting of electrospun nanofibers
with multichannel oriented and nanofibers aligned for peripheral
regeneration. Our bioactive silk tubes thus represent a new and
promising technique towards the creation of a biocompatible nerve
guidance conduit.
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