Young Blood Revitalizes the Aging Brain
Lab grown here:
- ↵* Corresponding author; email: drjthon@gmail.com
Key points
- We have developed a biomimetic microfluidic platelet bioreactor that recapitulates bone marrow and blood vessel microenvironments.
- Application of shear stress in this bioreactor triggers physiological proplatelet production, and platelet release.
Abstract
Platelet
transfusions total >2.17 million apheresis-equivalent units/year in
the United States and are derived entirely from human donors despite
clinically significant immunogenicity, associated risk of sepsis, and
inventory shortages due to high demand and 5-day shelf life. To take
advantage of known physiological drivers of thrombopoiesis we have
developed a microfluidic human platelet bioreactor that recapitulates
bone marrow stiffness, extracellular matrix composition, micro-channel
size, hemodynamic vascular shear stress, and endothelial cell contacts,
and supports high-resolution live-cell microscopy and quantification of
platelet production. Physiological shear stresses triggered proplatelet
initiation, reproduced ex vivo bone marrow proplatelet production, and
generated functional platelets. Modeling human bone marrow composition
and hemodynamics in vitro obviates risks associated with platelet
procurement and storage to help meet growing transfusion needs.
No comments:
Post a Comment