Deans' stroke musings: A “turn-on” fluorescent microbead sensor for detecting nitric oxide

Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Monday, December 22, 2014

A “turn-on” fluorescent microbead sensor for detecting nitric oxide

Since nitric oxide is so helpful for us it would be good to know that the breathing exercises we are doing to produce it actually works.

Breathing Exercises for Your Heart

How else is your doctor making sure that the breathing protocol that was prescribed for you is working the way it is supposed to?
http://www.dovepress.com/articles.php?article_id=19691

Authors Yang LH, Ahn DJ, Koo E
Published Date December 2014 Volume 2015:10 Pages 115—123
DOI http://dx.doi.org/10.2147/IJN.S74924
Received 25 September 2014, Accepted 18 November 2014, Published 19 December 2014
Approved for publication by Dr Thomas J. Webster

Lan-Hee Yang,^1,2 Dong June Ahn,³ Eunhae Koo¹

¹Advanced Materials Convergence Division, Korea Institute of Ceramic Engineering and Technology, Seoul, Republic of Korea; ²Department of Biomicrosystem Technology, Korea University, Seoul, Republic of Korea; ³Departments of Biomicrosystem Technology, Chemical & Biological Engineering, KU-KIST Graduate School, Korea University, Seoul, Republic of Korea

Abstract: Nitric oxide (NO) is a messenger molecule involved in numerous physical and pathological processes in biological systems. Therefore, the development of a highly sensitive material able to detect NO in vivo is a key step in treating cardiovascular and a number of types of cancer-related diseases, as well as neurological dysfunction. Here we describe the development of a fluorescent probe using microbeads to enhance the fluorescence signal. Microbeads are infused with the fluorophore, dansyl-piperazine (Ds-pip), and quenched when the fluorophore is coordinated with a rhodium (Rh)-complex, ie, Rh₂(AcO^-)₄(Ds-pip). In contrast, they are able to fluoresce when the transition-metal complex is replaced by NO. To confirm the “on/off” mechanism for detecting NO, we investigated the structural molecular properties using the Fritz Haber Institute ab initio molecular simulations (FHI-AIMS) package. According to the binding energy calculation, NO molecules bind more strongly and rapidly with the Rh-core of the Rh-complex than with Ds-pip. This suggests that NO can bond strongly with the Rh-core and replace Ds-pip, even though Ds-pip is already near the Rh-core. However, the recovery process takes longer than the quenching process because the recovery process needs to overcome the energy barrier for formation of the transition state complex, ie, NO-(AcO^-)₄-(Ds-pip). Further, we confirm that the Rh-complex with the Ds-pip structure has too small an energy gap to give off visible light from the highest unoccupied molecular orbital/lowest unoccupied molecular orbital energy level.