Deans' stroke musings: BRAIN-DERIVED NEUROTROPHIC FACTOR DELIVERED TO THE BRAIN USING POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IMPROVES NEUROLOGICAL AND COGNITIVE OUTCOME IN MICE WITH TRAUMATIC BRAIN INJURY

Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Tuesday, June 14, 2016

BRAIN-DERIVED NEUROTROPHIC FACTOR DELIVERED TO THE BRAIN USING POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IMPROVES NEUROLOGICAL AND COGNITIVE OUTCOME IN MICE WITH TRAUMATIC BRAIN INJURY

More research needed in humans which won't occur because we have NO stroke leadership following up promising research. Oh well, Who cares? Certainly not our fucking failures of stroke associations.
http://www.tandfonline.com/doi/abs/10.1080/10717544.2016.1199609

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DOI:: 10.1080/10717544.2016.1199609

Igor Khalin^a^*, Renad Alyautdin^b, Tin Wui Wong^c, Justin Gnanou^a, Ganna Kocherga^d & Jörg Kreuter^e

Publishing models and article dates explained

Received: 15 May 2016
Accepted: 7 Jun 2016
Accepted author version posted online: 08 Jun 2016
Published online: 08 Jun 2016

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Abstract

Currently, traumatic brain injury (TBI) is the leading cause of death or of disabilities in young individuals worldwide. The multi-complexity of its pathogenesis as well as impermeability of the blood-brain barrier (BBB) make the drug choice and delivery very challenging. The brain-derived neurotrophic factor (BDNF) regulates neuronal plasticity, neuronal cell growth, proliferation, cell survival, and long term memory. However, its short half-life and low BBB permeability are the main hurdles to be an effective therapeutic for TBI. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated by surfactant can enable the delivery of a variety of molecules across the BBB by receptor-mediated transcytosis. This study examines the ability of PLGA nanoparticles coated with poloxamer 188 (PX) to deliver BDNF into the brain and neuroprotective effects of BNDF in mice with TBI. C57bl/6 mice were subjected to weight-drop closed head injuries under anesthesia. Using enzyme-linked immunosorbent assay, we demonstrated that the intravenous (IV) injection of nanoparticle-bound BDNF coated by PX (NP-BDNF-PX) significantly increased BDNF levels in the brain of sham-operated mice (p < 0.001) and in both ipsi- (p < 0.001) and contralateral (p < 0.001) parts of brain in TBI mice compared to controls. The present study also showed using the passive avoidance (PA) test, that IV injection of NP-BDNF-PX 3 hours post-injury prolonged the latent time in mice with TBI thereby reversing cognitive deficits caused by brain trauma. Finally, neurological severity score test demonstrated that our compound efficiently reduced the scores at day 7 after the injury indicating the improvement of neurological deficit in animals with TBI. This study shows that PLGA nanoparticles coated with PX effectively deliver BDNF into the brain, and improve neurological and cognitive deficits in TBI mice, thereby providing a neuroprotective effect.