Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia

We will never know if this might help stroke survivors because we have no fucking stroke leaders that followup promising research with human clinical trials. You'll be screwed forever until we destroy the existing stroke leadership.

Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia

Wang-shu Xu¹, Xuan Sun², Cheng-guang Song³, Xiao-peng Mu⁴, Wen-ping Ma⁵, Xing-hu Zhang⁶, Chuan-sheng Zhao M.D. ^4
1 Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province; Neuroinfection and Neuroimmunology Center, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
² Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
³ Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang; Department of Neurology, Benxi Central Hospital of China Medical University, Benxi, Liaoning Province, China
⁴ Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China
⁵ Department of Medical Genetics, School of Basic Medicine, Peking University, Beijing, China
⁶ Neuroinfection and Neuroimmunology Center, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Date of Acceptance	25-Feb-2016
Date of Web Publication	1-Jun-2016

Correspondence Address:
Chuan-sheng Zhao
Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province
China

Source of Support: None, Conflict of Interest: None
DOI: 10.4103/1673-5374.182700

Abstract

Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 µg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia.

Keywords: nerve regeneration; cerebral ischemia; bumetanide; Na ⁺ -K ⁺ -2Cl^- cotransporter 1; hippocampal dentate gyrus; neurogenesis; neural precursor cells; dendritic development; cognitive function; neural regeneration

How to cite this article: Xu Ws, Sun X, Song Cg, Mu Xp, Ma Wp, Zhang Xh, Zhao Cs. Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia. Neural Regen Res 2016;11:745-51