Deans' stroke musings

Changing stroke rehab and research worldwide now.Time is Brain!Just think of all the trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 493 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It's quite disgusting that this information is not available from every stroke association and doctors group.
My back ground story is here:http://oc1dean.blogspot.com/2010/11/my-background-story_8.html

Monday, April 3, 2017

The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Cul ture in Young Mice

BDNF is good for our recovery. What the hell is your doctor doing to ensure your levels are good and are getting enough?

The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice

 

Emma Kamelia
1
, Hadiyat Miko
1, *
, Marni Br Karo
2
, Andi Asadul Islam
3
, Mochammad Hatta
3
1
Department of Health, Polytechnic of Health Tasikma
laya, Tasikmalaya, West Java, Indonesia
2
Midwifery Program of Medistra Health Higher School,
Jakarta, Indonesia
3
Molecular Biology and Immunology Laboratory for Infe
ction Diseases, Faculty of Medicine, Hasanuddin Uni
versity Makassar, Indonesia
Email address:
drgmiko@yahoo.com (H. Miko)
*
Corresponding author
To cite this article:
Emma Kamelia, Hadiyat Miko, Marni Br Karo, Andi Asad
ul Islam, Mochammad Hatta. The Effect of Leaf Extra
ct of
Centella Asiatica
on
Neurogenesis and Bdnf Level in Hippocampus Cell Cultur
e in Young Mice.
Clinical Neurology and Neuroscience.
Vol. 1, No. 2, 2017, pp. 14-19. doi: 10.11648/j.cnn
.20170102.11
Received
: January 5, 2017;
Accepted
: January 21, 2017;
Published
: March 15, 2017
Abstract:
In many world, research of
centella asiatica (CA) leaf extracts are used to treat neuronal disease with stem cell or
cell line, but in the present study we try to make from hippocampus tissue culture.
Methods
: Young female mice dowley spraque types age of 2 weeks, as many as eighteen (18) tails each taken from its hippocampal tissue then cultured using a medium
Roswell Park Memorial Institute (RPMI). Eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.25 ug / ml (CA A) and eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.50 ug / ml (CA B), where as eighteen (18) other nerve cell cultures not exposed (control). Then calculated the number of cells per field view and BDNF levels measured in the culture of the
three populations.
Results
: (1) Anumber of larger nerve cells in cell cultures of hippocampal tissue of young rats were fed by CA extracts dose of 0.50 ug / ml compared to 0.25 ug / ml and the control group (p <0.05); that new neurons plays an important role in sinaptogenesis circuit in order to improve the integration function of neurons and synapses that still exist between new and mature neurons in the hippocampus [1
].
Conclusion:
(2)
Levels of BDNF CA dose of 0.50 ug / ml higher than 0.25 mg/ml CA doses but lower than in the controls (p <0.05), that the effect of the extract CA neurogenesis does occur in particular BDNF through the expression of BDNF, the possibility of glutamate in CA extracts is not able to increase the level of GABA in the brain due to activation of GABA synapses on a young neuron is depolarization where the concentration of intracellular Cl ion is high because channel expression Cl exporter KCC2 which slowed (delayed) [2].

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