The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Cul ture in Young Mice

BDNF is good for our recovery. What the hell is your doctor doing to ensure your levels are good and are getting enough?

The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice

 

Emma Kamelia

1

, Hadiyat Miko

1, *

, Marni Br Karo

2

, Andi Asadul Islam

3

, Mochammad Hatta

3

1

Department of Health, Polytechnic of Health Tasikma

laya, Tasikmalaya, West Java, Indonesia

2

Midwifery Program of Medistra Health Higher School,

Jakarta, Indonesia

3

Molecular Biology and Immunology Laboratory for Infe

ction Diseases, Faculty of Medicine, Hasanuddin Uni

versity Makassar, Indonesia

Email address:

drgmiko@yahoo.com (H. Miko)

*

Corresponding author

To cite this article:

Emma Kamelia, Hadiyat Miko, Marni Br Karo, Andi Asad

ul Islam, Mochammad Hatta. The Effect of Leaf Extra

ct of

Centella Asiatica

on

Neurogenesis and Bdnf Level in Hippocampus Cell Cultur

e in Young Mice.

Clinical Neurology and Neuroscience.

Vol. 1, No. 2, 2017, pp. 14-19. doi: 10.11648/j.cnn

.20170102.11

Received

: January 5, 2017;

Accepted

: January 21, 2017;

Published

: March 15, 2017

Abstract:

In many world, research of

centella asiatica (CA) leaf extracts are used to treat neuronal disease with stem cell or

cell line, but in the present study we try to make from hippocampus tissue culture.

Methods

: Young female mice dowley spraque types age of 2 weeks, as many as eighteen (18) tails each taken from its hippocampal tissue then cultured using a medium

Roswell Park Memorial Institute (RPMI). Eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.25 ug / ml (CA A) and eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.50 ug / ml (CA B), where as eighteen (18) other nerve cell cultures not exposed (control). Then calculated the number of cells per field view and BDNF levels measured in the culture of the

three populations.

Results

: (1) Anumber of larger nerve cells in cell cultures of hippocampal tissue of young rats were fed by CA extracts dose of 0.50 ug / ml compared to 0.25 ug / ml and the control group (p <0.05); that new neurons plays an important role in sinaptogenesis circuit in order to improve the integration function of neurons and synapses that still exist between new and mature neurons in the hippocampus [1

].

Conclusion:

(2)

Levels of BDNF CA dose of 0.50 ug / ml higher than 0.25 mg/ml CA doses but lower than in the controls (p <0.05), that the effect of the extract CA neurogenesis does occur in particular BDNF through the expression of BDNF, the possibility of glutamate in CA extracts is not able to increase the level of GABA in the brain due to activation of GABA synapses on a young neuron is depolarization where the concentration of intracellular Cl ion is high because channel expression Cl exporter KCC2 which slowed (delayed) [2].