I like the last lines, they would have helped me tremendously. But this might require the knowledge of what white matter damage you have vs. gray matter damage.
Background
Erythropoietin
(EPO), a hematopoietic cytokine, enhances neurogenesis and angiogenesis
during stroke recovery. In the present study, we examined the effect of
EPO on oligodendrogenesis in a rat model of embolic focal cerebral
ischemia.
Methodology and Principal Findings
Recombinant
human EPO (rhEPO) at a dose of 5,000 U/kg (n = 18) or saline (n = 18)
was intraperitoneally(
In mammals, the serous membrane lining the cavity of the abdomen and that is folded over the viscera.) administered daily for 7 days starting 24 h after
stroke onset. Treatment with rhEPO augmented actively proliferating
oligodendrocyte progenitor cells (OPCs) measured by NG2 immunoreactive
cells within the peri-infarct white matter and the subventricular zone
(SVZ), but did not protect against loss of myelinating oligodendrocytes
measured by cyclic nucleotide phosphodiesterase (CNPase) positive cells 7
days after stroke. However, 28 and 42 days after stroke,
treatment with
rhEPO significantly increased myelinating oligodendrocytes and
myelinated axons within the peri-infarct white matter. Using lentivirus
to label subventricular zone (SVZ) neural progenitor cells, we found
that in addition to the OPCs generated in the peri-infarct white matter,
SVZ neural progenitor cells contributed to rhEPO-increased OPCs in the
peri-infarct area. Using bromodeoxyuridine (BrdU) for birth-dating
cells, we demonstrated that myelinating oligodendrocytes observed 28
days after stroke were derived from OPCs. Furthermore, rhEPO
significantly improved neurological outcome 6 weeks after stroke. In
vitro, rhEPO increased differentiation of adult SVZ neural progenitor
cells into oligodendrocytes and enhanced immature oligodendrocyte cell
proliferation.
Conclusions
Our
in vivo and in vitro data indicate that EPO amplifies stroke-induced
oligodendrogenesis that could facilitate axonal re-myelination and lead
to functional recovery after stroke.
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