- An aggregate of 5826 adults from the National Health and Nutrition Examination Survey (NHANES) were studied cross-sectionally.
- Utilizing the quantitative polymerase chain reaction method, telomere length was compared to standard reference DNA.
- After that, caffeine intake from foods and beverages and coffee consumption were measured utilizing a validated, multi-pass, computer-assisted, 24-h recall system administered by NHANES interviewers.
- The following covariates were controlled: age, gender, race, marital status, education, housing, smoking, BMI, physical activity, alcohol use, and coffee intake (or caffeine consumption).
- The results of this study showed that caffeine consumption was inversely related to telomere length (F = 15.1, P = 0.0005).
- For each 100 mg of caffeine consumed, telomeres were 35.4 base pairs shorter, after adjusting for the covariates.
- For each 100 mg of caffeine consumed among coffee drinkers only, telomeres were 36.7 base pairs shorter (F = 9.0, P = 0.0054), and among non-coffee drinkers only, 40.0 base pairs shorter (F = 8.5, P = 0.0067).
- Findings revealed that coffee intake was positively related to telomere length (F = 12.6, P = 0.0013), independent of the covariates.