Followup needed to create a stroke protocol out of this.
Exosome mediated delivery of miR-124 promotes neurogenesis after ischemia
Title:
Exosome mediated delivery of
miR-124 promotes neurogenesis after ischemia
Running title:
Exosomal delivery of miR-124 promotes neurogenesis
Authors:
Jialei Yang
a,b,1
, Xiufen Zhang
b,1
, Xiangjie Chen
c
, Lei Wang
a
*
, Guodong
Yang
b
*
Authors affiliations:
a
Department of Neurology, New
Era Stroke Care and Re
search Institute, The General
Hospital of the PLA Rocket
Force, 16 Xinjiekouwai A
venue, Beijing 100088, China.
b
Department of Biochemistry
and Molecular Biology, S
chool of Basic Medicine, The
Fourth Military Medical
University, 169 Changlexi R
oad, Xi’an, Shaanxi 710032,
China.
c
Department of Mathematics,
Southeast University, Na
njing 211189, China.
1
These authors contribute
equally to the work.
*Correspondence should be
addressed to Dr. Guodong
Yang (yanggd@fmmu.edu.cn.)
and Dr. Lei Wang
(hellowanglei068@163.com)
*Corresponding author:
Dr. Guodong Yang
Department of Biochemistry
and Molecular Biology, S
chool of Basic Medicine,
Fourth Military Medical University,
169 Chang Le Xi
Road, Xi’an, Shaanxi 710032,
China.
Tel: +86-29-84774516
Fax: +86-29-84774937
Email: yanggd@fmmu.edu.cn.
Dr. Lei Wang
Department of Neurology, New
Era Stroke Care and Re
search Institute, The Second
Artillery General Hospital,
Chinese People’s Libera
tion Army, 16 Xinjiekouwai
Avenue, Beijing 100088,
China.
Tel:+86-10-66343682
Email:
hellowanglei068@163.com
Abstract
The intrinsic ability of
neurogenesis after stroke has been proven weak, which
results in insufficient repair of
injury in the nerve system. Recent studies suggest
multiple microRNAs (miRNAs) are
involved in the neuroremodeling process. Targeted miRNAs delivery for
amplification of neurogenesis is promising in promoting the prognosis after ischemia.
Here we showed that modified exosomes, with rabies
virus glycoprotein (RVG) fused to
exosomal protein lysosome-associated membrane glycoprotein 2b (Lamp2b),
could efficiently deliver miR-124 to the infarct site. Systemic administration of
RVG-exosomes loaded with miR-124 promoted cortical neural progenitors to obtain
neuronal identity and protect against ischemic
injury by robust cortical neurogenesis.
Our study suggests that RVG-exosomes can be utilized therapeutically for the
targeted delivery of gene drugs to the brain, thus
having great potential for clinical
applications.
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