We are going to need neurite outgrowth so our researchers should be able to figure out how to use this in our neurogenesis and neuroplasticity.
http://www.ncbi.nlm.nih.gov/pubmed/22617741
Abstract
Designing synthetic microenvironments for cellular
investigations is a very active area of research at the crossroads of
cell biology and materials science. The present work describes the
design and functionalization of a three-dimensional (3D) culture support
dedicated to the study of neurite outgrowth from neural cells. It is
based on a dense self-assembled collagen matrix stabilized by
100-nm-wide interconnected native fibrils without chemical crosslinking.
The matrices were made suitable for cell manipulation and direct
observation in confocal microscopy by anchoring them to traditional
glass supports with a calibrated thickness of ∼50μm. The matrix
composition can be readily adapted to specific neural cell types,
notably by incorporating appropriate neurotrophic growth factors. Both
PC-12 and SH-SY5Y lines respond to growth factors (nerve growth factor
and brain-derived neurotrophic factor, respectively) impregnated and
slowly released from the support. Significant neurite outgrowth is
reported for a large proportion of cells, up to 66% for PC12 and 49% for
SH-SY5Y. It is also shown that both growth factors can be chemically
conjugated (EDC/NHS) throughout the matrix and yield similar proportions
of cells with longer neurites (61% and 52%, respectively). Finally,
neurite outgrowth was observed over several tens of microns within the
3D matrix, with both diffusing and immobilized growth factors.
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