Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Tuesday, July 25, 2023

A Mild Dose of Aspirin Promotes Hippocampal Neurogenesis and Working Memory in Experimental Ageing Mice

I'm doing a 325 aspirin for blood thinning. WHOM DO I ASK IF THIS IS THE RIGHT AMOUNT TO GENERATE HUMAN NEUROGENESIS?

A Mild Dose of Aspirin Promotes Hippocampal Neurogenesis and Working Memory in Experimental Ageing Mice

1,3,*
1
Laboratory of Stem Cells and Neuroregeneration, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India
2
Molecular Neuro-Gerontology Laboratory, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India
3
University Grants Commission-Faculty Recharge Programme (UGC-FRP), New Delhi 110002, India
*
Author to whom correspondence should be addressed.
Brain Sci. 2023, 13(7), 1108; https://doi.org/10.3390/brainsci13071108
Received: 14 June 2023 / Revised: 16 July 2023 / Accepted: 17 July 2023 / Published: 21 July 2023
(This article belongs to the Special Issue Neuroregenerative Plasticity in Health and Disease)

Abstract

Aspirin has been reported to prevent memory decline in the elderly population. Adult neurogenesis in the hippocampus has been recognized as an underlying basis of learning and memory. This study investigated the effect of aspirin on spatial memory in correlation with the regulation of hippocampal neurogenesis and microglia in the brains of ageing experimental mice. Results from the novel object recognition (NOR) test, Morris water maze (MWM), and cued radial arm maze (cued RAM) revealed that aspirin treatment enhances working memory in experimental mice. Further, the co-immunohistochemical assessments on the brain sections indicated an increased number of doublecortin (DCX)-positive immature neurons and bromodeoxyuridine (BrdU)/neuronal nuclei (NeuN) double-positive newly generated neurons in the hippocampi of mice in the aspirin-treated group compared to the control group. Moreover, a reduced number of ionized calcium-binding adaptor molecule (Iba)-1-positive microglial cells was evident in the hippocampus of aspirin-treated animals. Recently, enhanced activity of acetylcholinesterase (AChE) in circulation has been identified as an indicative biomarker of dementia. The biochemical assessment in the blood of aspirin-treated mice showed decreased activity of AChE in comparison with that of the control group. Results from this study revealed that aspirin facilitates hippocampal neurogenesis which might be linked to enhanced working memory.

Graphical Abstract

1. Introduction

Aspirin is one of the most widely used generic non-steroidal anti-inflammatory drugs (NSAIDs) in the treatment regime of pain and fever [1,2]. Regular intake of aspirin provides preventive measures against various ageing-associated diseases including cardiovascular illness, cerebral stroke, thrombosis, and cancer [3]. The blockade of the cyclooxygenase (COX)-2 enzyme responsible for the synthesis of inflammatory prostaglandins is a well-recognized mode of action for aspirin [4]. While various mood disorders, neurocognitive deficits, and psychiatric illnesses have been characterized by overexpression of COX-2 in association with, neuroinflammation and oxidative stress in the brain, pharmacological blockade of COX-2 has been considered a crucial neurotherapeutic intervention [5,6]. Among various COX-2 inhibitors, aspirin treatment has been speculated to enhance memory in the ageing population and mitigate neuropathogenesis in subjects with various brain diseases. While some correlative studies indicated that the association between aspirin treatment and memory is uncertain, ample scientific evidence strongly implies that aspirin prevents memory decline in elderly subjects [7,8]. Eventually, recent reports demonstrated that aspirin treatment considerably improves the synaptic plasticity in the cognitive centers of the experimental brains [9]. Moreover, the implementation of aspirin has been considered for the treatment regime of memory loss in ageing and neurodegenerative disorders including Alzheimer’s disease (AD) [10,11]. However, the underlying cellular mechanism through which aspirin modulates neuroplasticity responsible for neurocognitive measures remains unclear. Adult neurogenesis is an inimitable neuroregenerative process in which new functional neurons are continuously generated from neural stem cells (NSCs) in the hippocampus and subventricular zone (SVZ)olfactory bulb (OB) system of the brain [12,13,14]. Notably, the occurrence of neurogenesis in the hippocampus has been identified to provide the cellular basis of pattern separation, mood, spatial learning, and working memory in physiological conditions [15,16]. In contrast, memory loss upon ageing has been attributed to decreased hippocampal neurogenesis [17]. Moreover, neurodegenerative disorders have been characterized by impaired hippocampal neurogenesis due to chronic neuroinflammation accounting for various neurological deficits and dementia [12,13,18]. Therefore, targeting a centralized neuroinflammatory pathway such as COX signaling that interlinks different pathogenic mechanisms and suppresses the hippocampal neuroregenerative plasticity during ageing and neuropathogenic conditions has become an unmet therapeutic need for progressive memory loss. Considering its prominent COX inhibitory potential aspirin treatment can be presumed to be involved in the regulation of hippocampal neurogenesis in the brain that could facilitate a positive impact in boosting learning and memory. However, the scientific evidence for the effects of aspirin on the regulation of neuroregenerative potential of the brain associated with memory functions is highly limited. While aspirin has been consumed by a considerable number of individuals worldwide, understanding the neuropharmacological effects of aspirin on the modulation of neuroplasticity and neuroregenerative measures accountable for memory functions is an important scientific perusal at the preclinical state. Therefore, this study investigated the effect of aspirin on the regulation of hippocampal neurogenesis and neurocognitive behaviors in experimental ageing mice.
 
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