Optical excitation of organic semiconductors as a highly selective strategy to induce vascular regeneration and tissue repair

How close is your doctor to using this in your recovery?

Optical excitation of organic semiconductors as a highly selective strategy to induce vascular regeneration and tissue repair

Author links open overlay panelFrancescoMoccia^a

SharonNegri^aPawanFaris^aCarlottaRonchi^bFrancescoLodola^c

https://doi.org/10.1016/j.vph.2022.106998Get rights and content

Abstract

Therapeutic neovascularization represents a promising strategy to rescue the vascular network and restore organ function in cardiovascular disorders (CVDs), including acute myocardial infarction, heart failure, peripheral artery disease, and brain stroke. Endothelial colony forming cells (ECFCs), which are mobilized in circulation upon an ischemic insult, are commonly regarded as the most suitable cellular tool to achieve therapeutic neovascularization. ECFCs can be genetically or pharmacologically manipulated to enhance their vasoreparative potential by boosting specific pro-angiogenic signalling pathways. However, optical stimulation represents the most reliable approach to control cellular activity because of its high selectivity and unprecedented spatio-temporal resolution. Herein, we discuss a novel strategy to drive ECFC angiogenic activity in ischemic tissues by combining geneless optical excitation with photosensitive organic semiconductors. We describe how photoexcitation of the conducting polymer poly(3-hexylthiophene-2,5-diyl), also known as P3HT, stimulates extracellular Ca²⁺ entry through Transient Receptor Potential Vanilloid 1 (TRPV1) channels upon the production of hydrogen peroxide (H₂O₂) in the cleft between the nanomaterial and the cell membrane. H₂O₂-induced TRPV1-dependent Ca²⁺ entry stimulates ECFC proliferation and tube formation, thereby providing the proof-of-concept that photoexcitation of organic semiconductors may offer a reliable strategy to stimulate ECFCs-dependent neovascularization in CVDs.

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Physical activity improves long-term stroke outcome via endothelial nitric oxide synthase-dependent augmentation of neovascularization and cerebral blood flow.

And only from 2006, so what have your doctors been doing for the last 7 years? Amy has already told us how to create nitric oxide by ourselves by chanting. No self-prescription, you know how dangerous chanting is without a prescription from your doctor. You might start flying like those TM practitioners.
http://www.ncbi.nlm.nih.gov/pubmed/17038638

Abstract

Physical activity upregulates endothelial nitric oxide synthase (eNOS), improves endothelium function, and protects from vascular disease. Here, we tested whether voluntary running would enhance neovascularization and long-term recovery following mild brain ischemia. Wild-type mice were exposed to 30 minutes of middle-cerebral artery occlusion (MCAo) and reperfusion. Continuous voluntary running on wheels conferred long-term upregulation of eNOS in the vasculature and of endothelial progenitor cells (EPCs) in the spleen and bone marrow (BM). This was associated with higher numbers of circulating EPCs in the blood and enhanced neovascularization. Moreover, engraftment of TIE2/LacZ-positive BM-derived cells was increased in the ischemic brain. Four weeks after the insult, trained animals showed higher numbers of newly generated cells in vascular sites, increased density of perfused microvessels and sustained augmentation of cerebral blood flow within the ischemic striatum. Moreover, running conferred tissue sparing and improved functional outcome at 4 weeks. The protective effects of running on angiogenesis and outcome were completely abolished when animals were treated with a NOS inhibitor or the antiangiogenic compound endostatin after brain ischemia, and in animals lacking eNOS expression. Voluntary physical activity improves long-term stroke outcome by eNOS-dependent mechanisms related to improved angiogenesis and cerebral blood flow

POLYMERIC MATERIALS FOR NEOVASCULARIZATION

You will need to ask your doctor the difference between angiogenesis and neovascularization. A great dissertation.
https://www.ideals.illinois.edu/bitstream/handle/2142/44267/Ross_Devolder.pdf?sequence=1
Only 122 pages for your doctor.

Revascularization therapies have emerged as a promising strategy to treat various acute and

chronic wounds, cardiovascular diseases, and tissue defects

.

It is common to either administer proangiogenic growth factors, such as vascular endothelial growth factor (VEGF) or transplant cells that

endogenously express multiple proangiogenic factors.

Additionally, these strategies utilize a wide variety

of polymeric systems, including hydrogels and biodegradable plastics, to deliver proangiogenic factors in a sophisticated manner to maintain a sustained proangiogenic environment.

Despite some impressive results in rebuilding vascular networks, it is still

a challenging task to engineer mature and functional neovessels in

target tissues, because of the increasing complexities involved with neovascularization

applications

.

To resolve these challenges, this work aims to design a wide variety of proangiogenic biomaterial systems with tunable properties used for neovascularization therapies.

This thesis describes the design of several biomaterial systems used for the delivery of proangiogenic factors in neovascularization therapies, including: an electrospun/e

lectrosprayed biodegradable plastic patch used for directional blood vessel growth (Chapter 2), an alginate-

g

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pyrrolehydrogel system that biochemically stimulates cellular endogenous proangiogenic factor expression (Chapter 3), an enzyme catalyzed alginate

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g

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pyrrole hydrogel system for VEGF delivery (Chapter 4), an

enzyme activated alginate

-

g

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pyrrole hydrogel system with systematically controllable electrical and

mechanical properties (Chapter 5), and an alginate

-

g

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pyrrole hydrogel that enables the decoupled control

of electrical conductivity and mechanical rigidity and is use to electrically stimulate cellular endogenous proangiogenic factor expression (Chapter 6). Overall, the

biomaterial systems developed in this thesis

will be broadly useful for improving the quality of a wide array of molecular and cellular

based revascularization therapies