http://www.nature.com/articles/srep38997
- Scientific Reports 6, Article number: 38997 (2016)
- doi:10.1038/srep38997
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- Received:
- Accepted:
- Published online:
Abstract
Recent
studies have demonstrated microRNAs (miRNAs) and proteins are
beneficial to axon regeneration, which may be involved in
Electroacupuncture (EA) therapy against stroke. In this study, we aimed
to determine the pivotal role of PirB in EA-produced rehabilitation
against ischemic stroke; and to screen and investigate the potential
miRNAs directly regulating PirB expression. The results showed EA
treatment enhanced axon regeneration and new projections from the
corticospinal tract at 28 d after cerebral ischemic reperfusion injury
of rats. Then, we found EA decreased pirb mRNA and PirB protein
expression in the penumbra within 28 days after reperfusion. The
reduction of PirB expression facilitated neurite outgrowth after
oxygen-glucose deprivation injury. The miRNA microarray showed the level
of twenty kinds of miRNAs changed in the penumbra after EA
administration. The bioinformatics study and luciferase assay verified
miR-181b directly regulated pirb mRNA expression. EA increased
miR-181b levels in the penumbras, and improved neurobehavioral function
rehabilitation through miR-181b direct targeting of pirb mRNA to
regulate the expression of PirB, RhoA and GAP43. In conclusion, we
provide the first evidence that EA enhances rehabilitation against
stroke by regulating epigenetic changes to directly act on its targets,
such as the miR-181b/PirB/RhoA/GAP43 axis, which is a novel mechanism of
EA therapy.
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