Changing stroke rehab and research worldwide now.Time is Brain! trillions and trillions of neurons that DIE each day because there are NO effective hyperacute therapies besides tPA(only 12% effective). I have 523 posts on hyperacute therapy, enough for researchers to spend decades proving them out. These are my personal ideas and blog on stroke rehabilitation and stroke research. Do not attempt any of these without checking with your medical provider. Unless you join me in agitating, when you need these therapies they won't be there.

What this blog is for:

My blog is not to help survivors recover, it is to have the 10 million yearly stroke survivors light fires underneath their doctors, stroke hospitals and stroke researchers to get stroke solved. 100% recovery. The stroke medical world is completely failing at that goal, they don't even have it as a goal. Shortly after getting out of the hospital and getting NO information on the process or protocols of stroke rehabilitation and recovery I started searching on the internet and found that no other survivor received useful information. This is an attempt to cover all stroke rehabilitation information that should be readily available to survivors so they can talk with informed knowledge to their medical staff. It lays out what needs to be done to get stroke survivors closer to 100% recovery. It's quite disgusting that this information is not available from every stroke association and doctors group.

Friday, December 5, 2025

High-fructose diet induces depressive-like behaviors and short-term memory deficits through hippocampal neurogenesis impairment via neural stem cell dysfunction

 

Will your incompetent? doctor get the dietician to evaluate this and get the problem cases removed from all hospital locations? Even though this latest one is in mice?

Do you prefer your doctor, hospital and board of director's incompetence NOT KNOWING? OR NOT DOING?

Let's see how long your doctor has been incompetent!

High-fructose diet induces depressive-like behaviors and short-term memory deficits through hippocampal neurogenesis impairment via neural stem cell dysfunction

Abstract

Background

Neural stem cells (NSCs), crucial for brain function and repair, are disrupted by high-fructose diet (HFrD) in proliferation and survival, linking to neurogenesis impairment and neuropsychiatric risks. Mechanistic insights remain undefined.

Methods

Comprehensive behavioral assessments were conducted on HFrD mice, including the tail suspension test (TST) and sucrose preference test (SPT) for depressive-like behaviors, elevated plus maze (EPM) and open field test (OFT) for anxiety-like behaviors, as well as novel object recognition (NOR) and Morris water maze (MWM) for cognition. Hippocampal NSCs and newborn neurons were quantified by immunofluorescence, and fructose-treated NE-4C cells underwent RNA sequencing (RNA-seq) analysis coupled with measurements of proliferation, apoptosis and ferroptosis markers.

Results

HFrD mice showed depressive-like behaviors without anxiety-like behaviors, and exhibited impaired short-term memory in NOR but did not show impaired spatial memory in MWM. Decreased number of hippocampal NSCs and newborn neurons were observed, suggesting impaired neurogenesis. In vitro, fructose-treated NE-4c exhibited altered gene expression profiles, with PCA showing distinct clustering between treated and control groups. Further analysis (GO, KEGG, GSEA) indicated enrichment in energy metabolism pathways, including mitochondrial ATP synthesis (e.g., downregulated ATP5E, ATP5H). Consistently, intracellular ATP levels were elevated, indicating metabolic dysregulation. Further experiments demonstrated that high fructose promoted NSC proliferation via p53/Wnt pathways (upregulated CyclinA2, CDK1) while concurrently inducing apoptosis (BAX, P53 upregulation) and ferroptosis (reduced GPX4, elevated ROS, and lipid peroxidation).

Conclusion

This study elucidates the mechanistic link between HFrD-induced metabolic disruption and NSC dysfunction, providing novel insights into the pathogenesis of fructose-associated neuropsychiatric disorders.

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