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Involvement of Proteasome and Endoplasmic Reticulum Stress in Neurodegeneration after Global Brain Ischemia
Preprint from
Research Square,
21 Oct 2022
DOI:
10.21203/rs.3.rs-2174277/v1 PPR: PPR560802
Preprint
This article is a preprint. It may not have been peer reviewed.
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Abstract
A
brief period of transient global brain ischemia leads to a selective
ischemic neurodegeneration associated with death of hippocampal CA1
pyramidal neurons days after reperfusion. The mechanism of such
selective and delayed neurodegeneration is still uncertain. The aim of
our work was to study an involvement of proteasome and endoplasmic
reticulum (ER) stress in ischemic neurodegeneration. We have performed
laser scanning confocal microscopy analysis of brain slices from control
and experimental animals that underwent global brain ischemia in
duration of 15 minutes and varying times of reperfusion. We have focused
on ubiquitin, PUMA that is proapoptotic protein of Bcl-2 family
overexpressed in response to both proteasome and ER stress, and p53 that
controls expression of PUMA. We have also examined expression of HRD1,
E3 ubiquitin ligase that was shown to be overexpressed after ER stress.
Using cellular models of both proteasome and ER stress, we have examined
possible crosstalk between proteasome and ER stress. We demonstrate
that global brain ischemia is associated with an appearance of distinct
immunoreactivity of ubiquitin, PUMA and p53 in pyramidal neurons of CA1
layer of hippocampus 72 hours after ischemic insults. Such changes
corelate with a delay and selectivity of ischemic neurodegeneration.
Immunoreactivity of HRD1 observed in all investigated regions of rat
brain was transiently absent in both CA1 and CA3 neurones 24 hours after
ischemia in hippocampus that does not correlate with a delay and
selectivity of ischemic neurodegeneration. We do not document
significant crosstalk between proteasome and ER stress. Our results are
in favour of dysfunction of ubiquitin proteasome system and consequent
p53-induced expression of PUMA as the main mechanisms responsible for
selective and delayed degeneration of pyramidal neurons of hippocampal
CA1 layer in response to global brain ischemia.
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