I can't tell if this worked at all. What was the expected intervention result? Your mentors and senior researchers need to do a better job at describing the whole point of research.
Chitosan-Based Nanocapsules as a Delivery System of Hydrophobic Carnosic Acid, A Model Neuroprotective Drug
Joanna Odrobińska-Baliś
Monika Leśkiewicz 2 , Dorota Duraczyńska 1 , Szczepan Zapotoczny 3 , Władysław Lasoń 2 ,
Krzysztof Szczepanowicz 1
1 Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland; 2 Maj Institute of Pharmacology Polish
Academy of Science, Krakow, Poland; 3 Faculty of Chemistry, Jagiellonian University, Krakow, Poland
Correspondence: Joanna Odrobińska-Baliś, Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezpominajek 8,
Krakow, 30-239, Poland, Tel +48 12 6395128, Email joanna.odrobinska-balis@ikifp.edu.pl
Introduction:
Since the population of Europe is rapidly aging, the number of cases of neurodegenerative diseases sharply increases. One of the most significant limitations of current neurodegenerative disease treatment is the inefficient delivery of neuroprotective drugs to the affected part of the brain. One of the promising methods to improve the pharmacokinetic and pharmacodynamic properties of antioxidants is their encapsulation in nanocarriers.
Materials and Methods:
Encapsulation of carnosic acid into a chitosan-based nanoparticle system with ultrasound-assisted
emulsification process was developed. The physicochemical properties (size, stability, concentration of nanoparticles) of obtained nanocapsules were analyzed. Also, the cytotoxicity and neuroprotective effect in SH-SY5Y cells exposed to toxic concentration of H 2 O2 of the obtained nanoparticles were evaluated in vitro.
Results and Discussion:
The capsules with diameters between 90 and 150 nm and long-term stability were obtained. Cytotoxicity tests of empty capsules indicate that observed toxic effects were concentration dependent and lower concentrations (dilution above 500×) can be considered as safe for tested cells. Our study also indicates that encapsulation of carnosic acid decreased the cytotoxicity
of empty nanocapsules and can efficiently protect SH-SY5Y cells from factors causing cell destruction. In addition, the neuroprotective efficacy of carnosic acid loaded nanocapsules was also demonstrated in SH-SY5Y cells exposed to toxic concentration of H2 O 2.
The designed nanoparticles appear to possess sufficient biocompatibility to deserve their further evaluation in in vivo models.
Keywords: polysaccharide nanocapsules, modified chitosan, carnosic acid, neuroprotection
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