Targeting ASIC1a Promotes Neural Progenitor Cells Migration and Neurogenesis in Ischemic Stroke

Potentiating is NOT GOOD ENOUGH! Will you deliver results instead of 'potential'? My god, I'd fire the lot of you for not solving stroke, just twiddling your thumbs.

 Targeting ASIC1a Promotes Neural Progenitor Cells Migration and Neurogenesis in Ischemic Stroke

1
Targeting ASIC1a Promotes Neural Progenitor Cells Migration and Neurogenesis
in Ischemic Stroke
Hongfei Ge,1,3,# Tengyuan Zhou,1,3,# Chao Zhang,1,3,# Yupeng Cun,2 Weixiang Chen,1
Yang Yang,1 Qian Zhang,3 Huanhuan Li,1 Jun Zhong,1 Xuyang Zhang,1 Hua Feng,1,*
Rong Hu,1,3,*
1 Department of Neurosurgery and Key Laboratory of Neurotrauma, Southwest
Hospital, Third Military Medical University (Army Medical University), 400038
Chongqing, China.
2 Pediatric Research Institute, Ministry of Education Key Laboratory of Child
Development and Disorders, National Clinical Research Center for Child Health and
Disorders, Children’s Hospital of Chongqing Medical University, 400014 Chongqing,
China
3 Medical Research Center, Southwest Hospital, Third Military Medical University
(Army Medical University), 400038 Chongqing, China.
# These authors contributed equally to this work.
* Correspondence should be addressed to Rong Hu; huchrong@tmmu.edu.cn and Hua
Feng; fenghua8888@163.vip.com
Short Title：ASIC1a inhibition promotes NPCs migration and neurogenesisDownloaded from https://spj.science.org on March 25, 2023

2

Abstract

Cell replacement therapy using neural progenitor cells (NPCs) has been shown to
be an effective treatment for ischemic stroke(Really? Where is the protocol for that?). However, the therapeutic effect is
unsatisfactory due to the imbalanced homeostasis of the local microenvironment after
ischemia. Microenvironmental acidosis is a common imbalanced homeostasis in the
penumbra and could activate acid-sensing ion channels 1a (ASIC1a), a subunit of
proton-gated cation channels following ischemic stroke. However, the role of ASIC1a
in NPCs post-ischemia remains elusive. Here, our results indicated that ASIC1a was
expressed in NPCs with channel functionality, which could be activated by extracellular
acidification. Further evidence revealed that ASIC1a activation inhibited NPCs
migration and neurogenesis through RhoA signaling-mediated reorganization of
filopodia formation, which could be primarily reversed by pharmacological or genetic
disruption of ASIC1a. In vivo data showed that the knockout of the ASIC1a gene
facilitated NPCs migration and neurogenesis in the penumbra to improve behavioral
recovery after stroke. Subsequently, ASIC1a gain-of-function partially abrogated this
effect. Moreover, the administration of ASIC1a antagonists (Amiloride or Psalmotoxin
1) promoted functional recovery by enhancing NPCs migration and neurogenesis.
Together, these results demonstrate targeting ASIC1a is a novel strategy potentiating
NPCs migration toward penumbra to repair lesions following ischemic stroke, and even
for other neurological diseases with the presence of niche acidosis.Downloaded

Enhanced Rehab For Stroke Doubles Movement Recovery

Well then, write up a protocol AND DELIVER IT to all 10 million yearly stroke survivors  now and into the future.

on what works. Just this writeup is totally fucking useless, doctors and stroke hospitals do not read and implement research.

Enhanced Rehab For Stroke Doubles Movement Recovery

 Source: University of Texas at Dallas

A novel therapy technique invented by researchers at The University of Texas at Dallas has been shown in a pilot study to double the rate of upper limb recovery in stroke patients, a leap forward in treating the nearly 800,000 Americans who suffer strokes each year.

The results of the study, funded by UT Dallas spinoff company MicroTransponder of Austin, Texas, were published Sept. 27 in the journal Stroke.

The findings indicate that targeted plasticity therapy — which involves stimulation of the vagus nerve — paired with traditional motor-skill rehabilitation is not only safe, but also twice as effective as rehab alone.

Dr. Jane Wigginton, the chief medical officer at UT Dallas’ Texas Biomedical Device Center (TxBDC) and an associate professor of emergency medicine at UT Southwestern Medical Center, led the Dallas site of the clinical trial, which involved 17 people across the country who had suffered a stroke.

“Stroke is too common and too debilitating for us to tolerate the status quo,” Wigginton said. “Patients need a real solution so they can get back to fully living their lives.”

Dr. Michael Kilgard, associate director and chief science officer of the TxBDC, invented targeted plasticity therapy (TPT). Kilgard, who is also the Margaret Fonde Jonsson Professor in the School of Behavioral and Brain Sciences (BBS), said the study results further validate the theories that he and his colleagues based their TPT work on beginning in 2009.

“We set out to design an approach that could transform long-term care and restore quality of life to patients for whom that has thus far been impossible,” said Kilgard, who was not involved in the clinical trial. “These results show our method has immense potential. We’re excited about what this could mean for millions of stroke patients worldwide.”

Researchers affiliated with the TxBDC and BBS developed the therapy technique, which pairs physical movements with precisely timed vagus nerve stimulation (VNS) — electrical stimulus of the nerve via a device implanted on the nerve in the neck.

The vagus nerve controls the parasympathetic nervous system, overseeing many unconscious functions such as circulation and digestion. Stimulating the nerve initiates neural plasticity — reorganization of the brain’s circuitry. The idea behind TPT is that synchronizing VNS with movement accelerates plasticity in a damaged brain, and with it, recovery.

A stroke occurs when blood flow to the brain is interrupted because of a blockage or a ruptured blood vessel. Limb mobility can be affected when nerve cells are damaged. Such forms of brain trauma are often treated with rehabilitation that includes repeated movement of the affected limb in an effort to regain motor skills. The approach is thought to work by helping the brain reorganize.

Several studies of Kilgard’s technique in animal models have previously demonstrated that it is effective in recovering limb function after stroke. A small clinical trial in Europe also provided encouraging data for its potential use in humans.

In 2009, UT Dallas licensed its VNS technique as a stroke and tinnitus treatment to MicroTransponder, which sponsored the new double-blind, placebo-controlled study.

Neither the researchers nor the study subjects knew who was getting VNS stimulation and who was not.

Each study subject was a stroke patient whose stroke occurred between four months and five years prior to selection. After they had a VNS device implanted, the subjects received six weeks of in-clinic rehab followed by a home exercise program. About half were treated with active VNS while the rest received control VNS. All were assessed one, 30 and 90 days after therapy with a widely used, stroke-specific measure of performance impairment.

In addition to showing that the technique is safe, the researchers found that subjects receiving active VNS scored more than twice as high as control subjects at the 30- and 90-day intervals, opening the way for larger, more extensive clinical trials, Kilgard said. One such trial is in the recruitment phase and includes a study site in Dallas.

Other institutions involved in the study included The University of Texas Health Science Center at Houston; the University of Minnesota; the University of California, Irvine; the University of Glasgow; and Massachusetts General Hospital.

MicroTransponder had no responsibility for the analysis and interpretation of study data and had no responsibility for writing of the trial report or in the decision to submit the paper for publication. Kilgard is a shareholder and consultant for MicroTransponder Inc.

Repetitive peripheral magnetic stimulation improves severe upper limb paresis in early acute phase stroke survivors

Then write this up as a stroke protocol AND DELIVER IT to all 10 million yearly stroke survivors  now and into the future.

This printed research article is only the start of your job since we have fucking failures of stroke associations you can't dump the followup on them.

The latest here:

 Repetitive peripheral magnetic stimulation improves severe upper limb paresis in early acute phase stroke survivors

NeuroRehabilitation , Volume 46(4) , Pgs. 569-575.

NARIC Accession Number: J84280.  What's this?
ISSN: 1053-8135.
Author(s): Obayashi, Shigeru ; Takahashi, Rina.
Publication Year: 2020.
Number of Pages: 7.
Abstract: Study investigated the effects of repetitive peripheral magnetic stimulation (rPMS) on severe upper-extremity (UE) paresis during early acute phase of stroke. Nineteen participants with severe UE disability met the criteria. Ten subjects received 15–20 minutes of rPMS prior to standard care per session, while 9 age- and severity-matched subjects received two 20-minute sessions of standard care. Outcome measures included UE motor section of the Fugl-Meyer Motor Assessment Scale (FMA-UE), Wolf Motor Function Test (WMFT), and Box and Block Test (BBT). The rPMS group received treatment (average sessions: 7.8) after a median 9.2 days from stroke (16.5 sessions after 5 days for control). To adjust the different treatment durations, “progress rate” was defined as the gains of UE function scores divided by treatment duration. The progress rate was significantly different in FMA-UE and WMFT, but not in BBT. The findings suggest beneficial effects of rPMS on severe UE paresis during early acute phase of stroke.
Descriptor Terms: ACUTE CARE, EARLY INTERVENTION, ELECTRICAL STIMULATION, LIMBS, MOTOR SKILLS, PARALYSIS, STROKE.


Can this document be ordered through NARIC's document delivery service*?: Y.
Get this Document: https://content.iospress.com/articles/neurorehabilitation/nre203085.

Citation: Obayashi, Shigeru , Takahashi, Rina. (2020). Repetitive peripheral magnetic stimulation improves severe upper limb paresis in early acute phase stroke survivors.  NeuroRehabilitation , 46(4), Pgs. 569-575. Retrieved 9/19/2020, from REHABDATA database.
 

Sequencing bilateral robot-assisted arm therapy and constraint-induced therapy improves reach to press and trunk kinematics in patients with stroke

Then write this up as a stroke protocol AND DELIVER IT to all 10 million yearly stroke survivors and since this is chronic, those in the past, maybe 40-50 million. 

This printed research article is only the start of your job since we have fucking failures of stroke associations you can't dump the followup on them.

It has only been 4 years, WHERE THE FUCK IS THE PROTOCOL LOCATED? Survivors need to know. 

Sequencing bilateral robot-assisted arm therapy and constraint-induced therapy improves reach to press and trunk kinematics in patients with stroke

Journal of NeuroEngineering and Rehabilitation
 (2016) 13:31
DOI 10.1186/s12984-016-0138-5

Yu-wei Hsieh 1, 

Rong-jiuan Liing 2, 

Keh-chung Lin 2,3, 

Ching-yi Wu 1*, 

Tsan-hon Liou 4, 

Jui-chi Lin 4,
and Jen-wen Hung 5

Abstract

Background:
 The combination of robot-assisted therapy (RT) and a modified form of constraint-induced therapy(mCIT) shows promise for improving motor function of patients with stroke. However, whether the changes of motor control strategies are concomitant with the improvements in motor function after combination of RT andmCIT (RT + mCIT) is unclear. This study investigated the effects of the sequential combination of RT + mCIT compared with RT alone on the strategies of motor control measured by kinematic analysis and on motor function and daily performance measured by clinical scales.
Methods:
 The study enrolled 34 patients with chronic stroke. The data were derived from part of a single-blinded randomized controlled trial. Participants in the RT + mCIT and RT groups received 20 therapy sessions (90 to105 min/day, 5 days for 4 weeks). Patients in the RT + mCIT group received 10 RT sessions for first 2 weeks and 10mCIT sessions for the next 2 weeks. The Bi-Manu-Track was used in RT sessions to provide bilateral practice of wrist and forearm movements. The primary outcome was kinematic variables in a task of reaching to press a desk bell.Secondary outcomes included scores on the Wolf Motor Function Test, Functional Independence Measure, and Nottingham Extended Activities of Daily Living. All outcome measures were administered before and after intervention.
Results:
 RT + mCIT and RT demonstrated different benefits on motor control strategies. RT + mCIT uniquely improved motor control strategies by reducing shoulder abduction, increasing elbow extension, and decreasing trunk compensatory movement during the reaching task. Motor function and quality of the affected limb was improved, and patients achieved greater independence in instrumental activities of daily living. Force generation at movement initiation was improved in the patients who received RT.
Conclusion:
 A combination of RT and mCIT could be an effective approach to improve stroke rehabilitation outcomes, achieving better motor control strategies, motor function, and functional independence of instrumental activities of daily living.
Trial registration:
 ClinicalTrials.gov. NCT01727648

Feasibility of combining multi-channel functional neuromuscular stimulation with weight-supported treadmill training

You can ask your doctor where the protocol for this is from 16 years ago. 

Feasibility of combining multi-channel functional neuromuscular stimulation with weight-supported treadmill training

Janis J. Daly*, Robert L. Ruff
 Department of Neurology, Case Western Reserve University School of Medicine, United States Louis Stokes Cleveland VA Medical Center, Research Service 151-W 10701 E. Blvd., Cleveland, OH 44106, United States
Received 13 April 2004; received in revised form 30 June 2004; accepted 2 July 2004Available online 27 August 2004

Abstract

More than 3 million stroke survivors live with residual disabilities and mobility deficits even after rehabilitation. Therefore, it is important to develop new, more effective, gait training methods. The purpose of this study was twofold: (1) testing the feasibility of combining multi-channel functional neuromuscular stimulation (FNS) using intramuscular (IM) electrodes and body weight supported treadmill training(BWSTT) for gait training; and (2) documenting the potential gait practice advantages afforded by combining FNS-IM and BWSTT. Eight subjects with gait deficits in the chronic phase (
N
12 months) were enrolled. Intramuscular electrodes were placed in the paretic hip abductors,knee flexors and extensors, and ankle dorsiflexors,  plantar flexors, and evertors. Subjects were treated with exercise and gait training using the combined technologies 1 1/2 h/week, four times/week, for 12 weeks. Feasibility was tested according to performance of the technologies,clinician skill factors, and subject satisfaction. Impairment, function, and quality of life were measured. Provision of practice for eight gait characteristics was catalogued. We found the following results for the combined technologies: (1) the combined technologies were safe and feasible; (2) clinicians required five training sessions to reach proficiency; (3) subjects were satisfied; (4) there were significant gains inimpairment and functional measures; (5) a greater number of gait practice characteristics were provided with the combined technologies than with either alone.
D
 2004 Elsevier B.V. All rights reserved.